Green and Simple synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial activity.

Here, we are reported the biosynthesis procedure to prepare silver nanoparticles with the aqueous plant extract of Perovskia abrotanoides as reduction and capping agents. Due to the therapeutic application of silver nanoparticles, entirely depend on the size and shape of nanoparticles, the control of them during the synthesis procedure is so important. Since, the synthesis factors for example silver ion concentration, the mass of plant extract, and reaction time and extraction temperature on the silver particles size were considered and optimized. Several analysis methods were used for characterization of silver NPs containing FT-IR and UV-Vis spectrophotometric, XRD and SEM. The results are showed that the mean size of silver particles is about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs are investigated by the minimum inhibitory concentration, minimum bactericidal concentration, Well-diffusion tests. The minimum inhibitory concentration/minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL) respectively. The antimicrobial activity study is displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.