In vivo influence of cyanobacterial toxins on enzyme activity and gene expression of protein phosphatases in Alfalfa (Medicago sativa).
کلید واژه ها
خلاصه
Irrigation of crop plants with surface water can be a threat if cyanobacterial toxins are present in the water. Cyanotoxins are known to cause adverse effects in plants. Microcystin (MC), a cyclic heptapeptide, with more than 70 structural variants, is a frequently occurring toxin. MC is a specific inhibitor of serine/threonine protein phosphatases 1 and 2A (PP1 and 2A), important regulatory enzymes in eukaryotic cells. Protein phosphatases consist of a catalytic subunit and one or more regulatory subunits. In Alfalfa several isoforms of the catalytic subunit of PP1 (MsPP1alpha, MsPP1beta, MsPP1gamma, MsPP1delta, MsPP1varepsilon) and PP2A (MsPP2A Calpha/beta/gamma) are known along with isoforms of the regulatory subunits of PP2A (MsPP2A Aalpha/beta, MsPP2A Balpha/beta). The in vivo effect of environmentally relevant concentrations of cyanobacterial components on the mRNA transcript level of the subunits of protein phosphatases 1 and 2A in Alfalfa (Medicago sativa) was examined using semi-quantitative RT-PCR. Plants were exposed for one week to 5 microg L(-1) microcystin-LR, microcystin-LW, okadaic acid and to cell-free cyanobacterial crude extracts from Microcystis aeruginosa containing 5 microg L(-1) microcystin-LR and a toxin-free crude extract from Synechocystis spp. The protein phosphatase activity in vivo was inhibited when exposed to toxins and crude extract containing microcystin-LR, no change was induced by Synechocystis crude extract. The gene expression of the MsPP1gamma subunit and the MsPP1varepsilon subunit was induced in plants exposed to MC-LW.