Interplay between oxidative damage, the redox status, and metabolic biomarkers during long-term fasting

Obesity and its related metabolic disorders, as well as infectious diseases like covid-19, are important health risks nowadays. We recently documented that long-term fasting improves metabolic health and enhanced the total antioxidant capacity. The present study investigated the influence of a 10-day fasting on markers of the redox status in 109 subjects. Reducing power, ABTS radical scavenging capacity, and hydroxyl radical scavenging capacity increased significantly, and indicated an increase of circulating antioxidant levels. No differences were detected in superoxide scavenging capacity, protein carbonyls, and superoxide dismutase when measured at baseline and after 10 days of fasting. These findings were concomitant to a decrease in blood glucose, insulin, HbA1c, total cholesterol, LDL and triglycerides as well as an increase in total cholesterol/HDL ratio. In addition, the well-being index as well as the subjective energy levels increased, documenting a good tolerability. We documented an interplay between redox and metabolic parameters, as lipid peroxidation baseline levels (TBARS) affected the ability of long-term fasting to normalize lipid levels. A machine learning model showed that a combination of antioxidant parameters measured at baseline predicted the efficiency of the fasting regimen to decrease LDL levels. In conclusion, we demonstrated that long-term fasting enhanced the endogenous production of antioxidant molecules, that act protectively against free radicals, and in parallel improved the metabolic health status. Our results suggest that the outcome of long-term fasting strategies could be depending on the baseline values of the antioxidative and metabolic status of subjects.

Keywords: antioxidant capacity; dysmetabolism; oxidative stress; weight loss.