Potential of Myriophyllum aquaticum for phytoremediation of water contaminated with tetracycline antibiotics and copper

Water pollution caused by antibiotics and heavy metals has attracted considerable concern, and efficient approaches are urgently needed for their removal. The objective of this study was to investigate the potential of Myriophyllum aquaticum for long-term phytoremediation of wastewater containing tetracycline (TC) antibiotics and copper. Seven hydroponic microcosms were constructed, spiked with tetracycline, oxytetracycline (OTC) and chlortetracycline (CTC) (300-30,000 μg/L), alone or simultaneously with Cu (II), and operated for 12 weeks. The TC removal efficiencies using the hydroponic microcosms here were commensurate or higher than those in previous studies. However, the Cu/TC ratio greatly affected the removal, accumulation of TCs by M. aquaticum, and plant growth. Low levels of Cu (II) (<1000 μg/L) promoted TC removal, but excessive Cu (II) (>10,000 μg/L) impeded it. Mass balance analysis showed that most TCs (45%-64% on average) accumulated in the roots of M. aquaticum. Plant biomass was correlated with the removal of COD, TN, TP, and NH₄⁺-N (p ≤ 0.05) but not with removal of the TCs. Proteobacteria, Bacteroidetes, and Fusobacteria were dominant in the microbial communities, but they showed little correlation with the TC removal. M. aquaticum can be employed as an effective means of TC removal from water. The co-existence of heavy metals should be considered when evaluating the removal potential of TCs in phytoremediation.

Keywords: Copper; Hydroponic microcosm; Myriophyllum aquaticum; Phytoremediation; Tetracyclines antibiotics.