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Human neutrophil responses to pathogenic Escherichia coli are receptor-specific and selectively augmented by recombinant human tumor necrosis factor-alpha.

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The effect of recombinant human tumor necrosis factor-alpha (rhTNF alpha) on neutrophil (PMNL) response to uropathogenic Escherichia coli was assessed. A strain expressing mannose-sensitive adhesins (type 1 fimbriae) stimulated significant primary granule, secondary granule, and leukotriene B4

Natural killer (NK) cell-derived hematopoietic colony-inhibiting activity and NK cytotoxic factor. Relationship with tumor necrosis factor and synergism with immune interferon.

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We characterize the natural killer (NK) cell colony-inhibiting activity (CIA) produced in supernatants from cultures of human peripheral blood lymphocytes (PBL) with NK-sensitive target cell lines, and study its relationship with NK cell-derived cytotoxic factor (NKCF). Using monoclonal antibodies

Experimental study for cancer diagnosis with positron-labeled fluorinated glucose analogs: [18F]-2-fluoro-2-deoxy-D-mannose: a new tracer for cancer detection.

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18F-2-fluoro-2-deoxy-D-glucose (18F-FDG) and 18F-2-fluoro-2-deoxy-D-mannose (18F-FDM) were tested as tumor diagnostic agents in a transplantable rat tumor and rabbit tumors. Tissue distribution studies in rats showed high tumor uptakes of both radiopharmaceuticals. The tumor uptake reached 2.65 +/-

Secretion of TNF-alpha by alveolar macrophages in response to Candida albicans mannan.

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Resident alveolar macrophages (AM phi) were tested for their ability to respond to Candida albicans mannan. AM phi were found to produce tumor necrosis factor alpha (TNF-alpha) in vitro in response to mannan stimulation. TNF-alpha secretion was measured using ELISA and L929B cellular cytotoxicity

Serum-mediated enhancement of TNF-alpha release by human monocytes stimulated with the yeast form of Candida albicans.

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The role of serum in the production of tumor necrosis factor-alpha (TNF-alpha) by human monocytes stimulated with yeast-form Candida albicans was studied. Pre-exposure of C. albicans to human pooled serum enhanced both TNF-alpha mRNA and cytokine secretion compared with C. albicans preincubated with

Antimicrobial activity of mannose binding lectin in grass carp (Ctenopharyngodon idella) in vivo and in vitro.

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Mannose-binding lectin (MBL) is a crucial pattern recognition receptor in the host innate immune system. Previously, we reported the biological function of Ctenopharyngodon idella MBL (CiMBL) in initiating the lectin pathway of the complement system. In the present study, we further explored its

Neutrophils Contribute to the Protection Conferred by ArtinM against Intracellular Pathogens: A Study on Leishmania major.

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ArtinM, a D-mannose binding lectin from Artocarpus heterophyllus, has immunomodulatory activities through its interaction with N-glycans of immune cells, culminating with the establishment of T helper type 1 (Th1) immunity. This interaction protects mice against intracellular pathogens, including

Functional consequences of the binding of gliadin to cultured rat mesangial cells: bridging immunoglobulin A to cells and modulation of eicosanoid synthesis and altered cytokine production.

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Oral immunization with gliadin (GLI) can induce immunoglobulin A mesangial deposits (IgA nephropathy [IgAN]) in mice. A role for GLI in human IgAN has been inferred from an association with celiac disease, increased serum anti-GLI IgA in patients with IgAN, and benefit from a gluten-free diet

First Report of Pectobacterium carotovorum subsp. carotovorum on Spathiphyllum wallisii in Argentina.

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Peace lily (Spathiphyllum wallisii Regel) is a popular ornamental potted plant in Argentina. During May of 2008 (austral autumn), necrotic lesions of unknown etiology were observed on S. wallisii in a nursery in Pontevedra (34°45'6″S, 58°42'42″W). Plants first showed water-soaked areas starting from

Peripheral membrane molecules of leukocytes and NK cytotoxicity.

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Some leukocyte effector cell-surface molecules movement toward the adjoining target cells takes place during the reaction of NK cytotoxicity (NK R). The majority of the moving molecules are usually anchored via a divalent-ion-dependent interaction (PMM-M2+). The released PMM-M2+ can interact also

A heteropolysaccharide purified from leaves of Ilex latifolia displaying immunomodulatory activity in vitro and in vivo

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A novel polysaccharide (ILP50-2) was extracted, isolated and purified from the leaves of Ilex latifolia Thunb. Its structure was characterized as a repeating unit consisting of α-L-Araf-(1→, →3)-α-L-Araf-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, β-D-Galp-(1→,

Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells.

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Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant. However, little was known about the polysaccharides from Dendrobium officinale leaves. Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water

Antioxidant and immunostimulatory activities of polysaccharides extracted from Tremella aurantialba mycelia.

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In the present study, the physiochemical and biological properties of crude mycelium polysaccharide (CMCP) and purified mycelium polysaccharide (MCP) extracted from the Tremella aurantialba mycelium were investigated. A series of physiochemical properties were determined, including the total sugar,

First Outbreak of Bacterial Leaf Spot Caused by Xanthomonas campestris on Canola in Argentina.

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Canola (Brassica napus) is an alternative crop to wheat in Argentina and ~16,000 ha are grown commercially in southern Buenos Aires Province. During 2003, experimental field plots of canola plants located at Agronomy Faculty, University of Buenos Aires, in Buenos Aires were severely damaged by a

Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells.

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In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 (g/mL); an extraction temperature of 72 °C; and an extraction time of 3 h.

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Herbal & Natural Medicine

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