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The surface coat of chylomicrons: lipid chemistry.

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Chylomicrons from the thoracic duct lymph of dogs fed corn oil were isolated by centrifugation and disrupted by either freezing and thawing or rotary evaporation and rehydration. A pellet, representing the surface coat, was isolated by centrifugation. Pellets isolated by freezing and thawing

A comparative study of the lipids of chylomicron membrane and fat core and of the lymph serum of dogs.

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Thoracic lymph was collected from 13 dogs fed corn oil and butterfat. The chylomicrons were isolated by centrifugation. The lipid composition of the fat core and the membrane of the chylomicron was compared to that of the surrounding lymph serum. The fat cores contained 90-96% triglyceride, 0.7-1.9%

Abnormalities of high density lipoproteins in abetalipoproteinemia.

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DETAILED STUDIES OF THE HIGH DENSITY LIPOPROTEINS FROM THREE PATIENTS WITH ABETALIPOPROTEINEMIA HAVE REVEALED THE FOLLOWING PRINCIPAL ABNORMALITIES: 1) High density lipoprotein 3 (HDL3) is reduced in both absolute and relative concentration, although HDL2 is present in normal amounts. 2) The

Long-term deficiency of essential fatty acids in rats and its effect on brain recovery.

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1. Nineteen-day old rats were fed an essential fatty acid deficient diet for 37 weeks, thereby making them essential fatty acid deficient for at least 24 weeks. The weights of their brains were 33% less than those of rats on control diets. 2. When the deficient rats were rehabilitated for ten weeks

Effects of dietary n-3 and n-6 polyunsaturated fatty acids on macrophage phospholipid classes and subclasses.

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This study examined the effects of n-3 and n-6 polyunsaturated fatty acid alimentation on murine peritoneal macrophage phospholipids. Mice were fed complete diets supplemented with either corn oil predominantly containing 18:2n-6, borage oil containing 18:2n-6 and 18:3n-6, fish/corn oil mixture

Recovery of brain from deficiency of essential fatty acids in rats.

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Rats were made visibly deficient in essential fatty acids by feeding a deficient diet and were then fed a diet containing 5.0 per cent by weight corn oil as a source of essential fatty acids. After 11-13 weeks on deficient diet the weights of the brain were 25% less than those of rats on control

Preparation of radiolabled tetracosa mono- and dienoic acid methyl esters from rat erythrocyte lipids by thin layer chromatography.

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An easy method of obtaining pure fatty acid methyl esters (FAME) of tetracosa mono- and dienoic acids (24:1, 24:2) using thin layer chromatography (TLC) is described. The total lipids isolated from rat erythrocytes were treated with methanolic-NaOH. Sphingomyelin was unaffected by this treatment and

Effects of dietary saturated fat on erucic acid induced myocardial lipidosis in rats.

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Male Sprague-Dawley rats were fed for one week diets containing 20% by weight fat/oil mixtures with different levels of erucic acid (22:1n-9) (approximately 2.5 or 9%) and total saturated fatty acids (approximately 8 or 35%). Corn oil and high erucic acid rapeseed (HEAR) oil were fed as controls.

In vitro and in vivo effects of unsaturated fatty acids on Schistosoma mansoni and S. haematobium lung-stage larvae.

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Incubation of Schistosoma mansoni lung-stage larvae in 90% corn oil for 6 hr was shown to elicit exposure of their, otherwise masked, apical membrane antigens to binding of anti-schistosome antibodies in the indirect membrane immunofluorescence test (IF). The possibility that unsaturated fatty acids

Effects of dietary fats on the fluidity of human high-density lipoprotein: influence of the overall composition and phospholipid fatty acids.

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The present study was undertaken to analyze whether the changes induced by dietary manipulations in the chemical composition of HDL, particularly in total phospholipids, phosphatidylcholine and sphingomyelin fatty acid composition, modified their fluidity. 12 healthy women, aged 26-49 years were

Dietary docosahexaenoic acid suppresses T cell protein kinase C theta lipid raft recruitment and IL-2 production.

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To date, the proximal molecular targets through which dietary n-3 polyunsaturated fatty acids (PUFA) suppress the inflammatory process have not been elucidated. Because cholesterol and sphingolipid-enriched rafts have been proposed as platforms for compartmentalizing dynamically regulated signaling

Dietary lipids differentially affect membranes from different areas of rooster sperm.

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The present work aimed to compare the effect of dietary flax with other oil sources on rooster sperm membranes and on semen characteristics. White Leghorn roosters (16 per diet) were fed 1 of 4 treatments: control diet (CON), or a diet containing corn oil (CORN), fish oil (FISH), or flax seed (FLAX)

Dietary (n-3) polyunsaturated fatty acids remodel mouse T-cell lipid rafts.

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In vitro evidence indicates that (n-3) polyunsaturated fatty acids (PUFA) suppress T-cell activation in part by displacing proteins from lipid rafts, specialized regions within the plasma membrane that play an important role in T-cell signal transduction. However, the ability of (n-3) PUFA to

Surface components of chylomicrons from rats fed glyceryl or alkyl esters of fatty acids: minor components.

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The lipid class, fatty acid and molecular species composition of the minor polar surface components of rat lymph chylomicrons were determined during absorption of menhaden oil and corn oil or of the corresponding fatty acid ethyl esters. In addition to the previously reported minor polar lipids

Nervonic acid is transferred from the maternal diet to milk and tissues of suckling rat pups.

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Three experiments were designed to investigate the metabolism of dietary nervonic acid (24:1n-9, NA) during reproduction in the rat. The first experiment determined the effect of early development on the sphingomyelin (SM) composition of rat heart and liver tissues. Rats were fed a standard chow

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Herbal & Natural Medicine

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