Antipyretic effect of phytol, possibly via 5KIR-dependent COX-2 inhibition pathway.

This study is aimed at the evaluation of antipyretic effect of PHY in yeast-induced hyperthermia rats. Additionally, possible mechanism of antipyretic action of PHY has been also studied by molecular docking study.Adult male Wistar albino rats were treated with PHY at 100, 150 and 200 mg/kg in 0.05% Tween-80 dissolved in 0.9% NaCl solution. PHY was also given at 200 mg/kg with ibuprofen (IBU) 12.5 mg/kg (p.o.) or paracetamol (PARA) 100 mg/kg (p.o.) to see the combined effect of PHY in animals. In silico study of PHY was performed against cyclooxygenase (COX) enzymes (COX-1 and -2) proteins.PHY exhibited the antipyretic effect in febrile rats in a dose and time dependent manner. PHY 200 mg/kg co-treated with IBU12.5 or PARA100 exhibited greater antipyretic effect than the PHY or NSAIDs individual groups. Data from the computational study reveal that 5KIR of COX-2 is the most efficient receptor protein to which PHY interacts.PHY attributed an antipyretic effect, possibly via 5KIR-dependent COX-2 inhibition pathway.