Formation of plantlets through somatic embryogeny in the Himalayan blue poppy, Meconopsis simplicifolia (Papaveraceae).
Avainsanat
Abstrakti
Exuberant and subculturable calli could be induced from only hypocotyl and leaf segments of ca 4-month-old seedlings of Meconopsis simplicifolia cultured on Murashige & Skoog's medium supplemented with 10(-6)M kinetin + 10(-5)M α -naphthalene acetic acid. Suspension cultures were initiated from the calli in a similar medium but with 10(-5)M 2,4-dichlorophenoxy acetic acid in place of α -naphthalene acetic acid. In ca 80% of the suspension cultures somatic embryos differentiated freely (80-85%) as well as on the surface of small clumps of tissue (15-20%). Somatic embryos that developed beyond heart-shaped stage were transferred to agar-solidified Murashige & Skoog's medium free of growth substances. When maintained in 10 h light and 14 h dark the somatic embryos developed into plantlets bearing cauline leaves. From seed sowing to raising normal plantlets via callus required 28 weeks; on average 80 plantlets were obtained from one explant in three passages.