Optimization of bioactive preservative coatings of starch nanocrystal and ultrasonic extract of sour lemon peel on chicken fillets.

Starch nanocrystal (S-NC) was produced after sulfuric acid hydrolysis of potato starch granules and then characterized by laser diffraction particle size analyzer, scanning electron microscopy (SEM) and X-ray diffraction (XRD). Response surface methodology (RSM) was applied to optimize S-NC (2-10%) concentration, sour lemon peel extract (SLPE, 2.5-12.5%) amount, mixing temperature (M-TE, 25-65 °C) and mixing time (M-TI,15-75 min) in the preparation of bioactive coating solutions to develop the high-quality chicken fillets during the cold-storage. The optimized conditions for achieving the highest DPPH inhibition percentage (89.14%), antibacterial activity (Staphylococcus aureus, 3.58-mm; Escherichia coli, 3.14-mm; Listeria monocytogenes, 2.31-mm and Salmonella enterica, 2.24-mm) and lightness value (77.82) and the lowest redness (6.69), yellowness (13.21) values and viscosity (27.5 mPa.s) were 4.0% S-NC, 5.62% SLPE, 51.17 °C M-TE and 43.29 min M-TI. Spraying the optimal coating solution on chicken fillets led to a significant improvement in their physicochemical, textural and sensory characteristics compared to the control during 12-day cold-storage.