Supercritical CO2 extract from needles of Pinus nigra ssp. laricio: combined analysis by GC, GC-MS and 13C NMR.

The chemical composition of the volatile concentrate prepared by supercritical CO(2) extraction of the needles of Pinus nigra Arnold ssp. laricio Poiret from Corsica was investigated using GC (RI), GC-MS and (13)C NMR spectroscopy. The major component was by far manoyl oxide (63%), a compound of potential interest for the perfumery and pharmaceutical industries. Consequently, the supercritical fluid extract of P. nigra ssp. laricio (yield 1.60%) could be considered as a source of this diterpene.