In vitro gastrointestinal stability, bioaccessibility and potential biological activities of betalains and phenolic compounds in cactus berry fruits (Myrtillocactus geometrizans)

Cactus berry (Myrtillocactus geometrizans) is a scarcely studied Mexican wild fruit. These fruits could contribute to reduce the risk of degenerative chronic diseases due to their bioactive profile. The aim of this work was to study the betalains and phenolic profile in cactus berry, their in vitro biological activities and gastrointestinal digestive stability and bioaccessibility. 43 metabolites were identified by HPLC-DAD-ESI-QTOF (8 betaxanthins, 8 betacyanins, 13 flavonoids, 6 phenolic acids). Phyllocactin and Isorhamnetin rhamnosyl-rutinoside (IG2) were the most abundant metabolites (5876 and 396 µg/g dw) which were also bioaccessible (16 and 21%, respectively). Pulps showed higher (p ≤ 0.05) antioxidant activity by the Oxygen Radical Absorbance Capacity (27 mM Trolox equivalents). The anti-hyperglycemic activity was highest (p ≤ 0.05) in peel and pulp tissues (85% α-glucosidase and 8% α-amylase inhibition). An 83% inhibition of hyaluronidase showed high anti-inflammatory activity. Cactus berry fruit should be considered a promising fruit candidate for a sustainable healthy diet.

Keywords: Betalains; Bioaccessibility; Bioactivity; Cactus berry; Garambullo; Gastrointestinal digestive stability; INFOGEST®; Isorhamnetin (Pub Chem CID: 5281654); Phenolic compounds; betanidin (Pub Chem CID: 2368); betanin (Pub CChem CID: 12300103); indicaxanthin Pub (Chem CID:6096870); phyllocactin (Pub Chem CID: 101056997); quercetin rhamnoside (Pub Chem CID: 5280459); quercetin rhamnoside (Pub Chem CID: 5481663); rutin (Pub Chem CID: 5481663).