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Sivu 1 alkaen 42 tuloksia
First Molecular Evidence of Citrus psorosis virus and Citrus viroid III from Citrus spp. in New Zealand.
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In December 2008, a collection of Citrus spp. in Kerikeri, New Zealand was surveyed for virus and viroid diseases. Symptoms characteristic of virus or viroid infection were not observed other than Citrus tristeza virus (CTV)-associated stem pitting when examined with the bark removed. Total RNA was
Preparation of extracts for electrophoresis from citrus leaves.
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Extracting proteins from vegetative tissues while maintaining good enzyme activity and electrophoretic resolution presents numerous problems due to the presence of phenols, quinones, proteases and other components released during cell disruption. To overcome this problem in Citrus leaves, an
Binding Citrus flavanones to human serum albumin: effect of structure on affinity.
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Much of the bioactivities of Citrus flavanones significantly appear to impact blood and microvascular endothelial cells. It is essential to investigate the interaction between Citrus flavanones and serum albumin to verify the effect of flavanone structures on the distribution and transportation in
Radical scavenging activity and oxidative modification of citrus dehydrin.
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Dehydrins are ubiquitous proteins produced by plants in response to water stress. Their functions, however, are not fully understood. The overexpression of Citrus unshiu Marcov. dehydrin (CuCOR19) enhanced cold tolerance in transgenic plants by reducing lipid peroxidation promoted by cold stress,
Binding of citrus flavanones and their glucuronides and chalcones to human serum albumin.
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Naringenin and hesperetin glycosides are the major polyphenols (flavanones) of citrus fruits and juices and are thought to participate in the cardioprotective effects of diets rich in plant products. Naringenin and hesperetin glucuronides (resulting from conjugation at the A- or B-ring) are the main
Computational and spectroscopic analysis of interaction between food colorant citrus red 2 and human serum albumin.
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The main aim of this work was to gain insight into the binding properties between a food colorant, citrus red 2 (CR), and human serum albumin (HSA), which is the predominant protein in blood plasma. Here, computer simulations and multiple spectroscopies were applied to predict and characterize the
Screening of furanocoumarin derivatives in citrus fruits by enzyme-linked immunosorbent assay.
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This paper reports a sensitive and specific enzyme-linked immunosorbent assay for screening of furanocoumarin derivatives as cytochrome P450 3A4 inhibitors in citrus fruits. Anti-6',7'-dihydroxybergamottin antibody was obtained by immunizing rabbits with 6',7'-dihydroxybergamottin conjugated with
Development of a dot-immunobinding assay for detection of citrus tristeza virus.
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The dot-immunobinding assay (DIBA) was adapted for detection of citrus tristeza virus (CTV) and compared with DAS-ELISA and DAS-indirect ELISA. DIBA was easy to perform and as sensitive as either ELISA procedure for CTV diagnosis. The entire test could be performed in 2-3 h using polyclonal
Elucidating the chemical and biochemical applications of Citrus sinensis mediated silver nanocrystal.
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Synthesis of nanoparticles using biodegradable source is safer and echo-friendly. Here we describe the synthesis of polycrystalline silver nanocrystals using Citrus sinensis acting both as reducing and capping agent. After exposing the silver ions to orange extract, rapid reduction of silver ions
Fermented Citrus Lemon Reduces Liver Injury Induced by Carbon Tetrachloride in Rats.
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Fermented lemon juice displays a variety of important biological activities, including anti-inflammatory and antioxidant capabilities. The aim of the present study is to investigate hepatic-protective effects of no-sugar-added fermented lemon juice (FLJ) for liver inflammation caused by carbon
Partial purification and properties of a cysteine protease from citrus red mite Panonychus citri.
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Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify and assess properties of a cysteine protease from the mites acting as a potentially pathogenic factor to
In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits.
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Many polyphenols bind proteins, therefore our research was focused on the potential of protein binding to polyphenols of investigated fruits and their health-related effects. The contents of polyphenols and related antioxidant activities of traditional, citrus and exotic fruits were compared. The
Citrus Extract Improves the Absorption and Utilization of Nitrogen and Gut Health of Piglets.
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The purpose of this study was to investigate the effects of citrus extract (CE) on plasma free amino acids, intestinal morphology and enzymes activity, fecal nitrogen and phosphorus emissions in piglets. The experiment was performed on 144 weaned piglets (Duroc × Landrace × Large White) divided into
Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco.
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Citrus ( Citrus unshiu Marcov.) dehydrin in response to chilling stress was overexpressed in tobacco ( Nicotiana tabacum L.), and the cold stress tolerance of transgenics at low temperature was analyzed. The freezing at -4 degrees C for 3 h of 24 independent lines indicated that a phenotype
Preparation and Self-Assembly Mechanism of Bovine Serum Albumin-Citrus Peel Pectin Conjugated Hydrogel: A Potential Delivery System for Vitamin C.
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In this study, a novel hydrogel (BSA-pectin hydrogel, BPH) was prepared via a self-assembly method by using the natural polymers of bovine serum albumin (BSA) and citrus peel pectin (pectin). The rheological properties and gel conformational structures were determined and showed that electrostatic
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