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Lectin binding to normal mucosa, leukoplakia and squamous cell carcinoma of the oral cavity.

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A panel of fifteen biotinylated lectins was used to study the saccharides associated with the epithelial component of normal and leukoplakic mucosa and squamous cell carcinoma, from a variety of locations in the oral cavity. Lectin binding heterogeneity was found between various sites of normal

Tissue binding patterns of lectins in premalignant and malignant lesions of the oral cavity.

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Lectins from the seeds of Jackfruit (Artocarpus integrifolia) and winged bean (Psophocarpus tetragonolobus) were isolated using an immobilized N-acetyl D-galactosamine column and conjugated to type VI horse radish peroxidase. The purified conjugate was used for the study of tissue specificities

Bacterial translocation in D-galactosamine-treated rats in a burn model.

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Bacteria and endotoxins can pass through the gut barrier under certain conditions. This process of bacterial translocation (BT) may occur after thermal injury in animals and is thought to play a role in the pathogenesis of septic complications in severely burned patients. The current study was

Influence of liver disease on phenolsulfonphthalein absorption from liver surface to examine possibility of direct liver surface application for drug targeting.

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We have examined the influence of liver disease on drug absorption from the liver surface membrane, regarded as the first barrier for drug targeting to the liver. The main purpose of this study is to examine the possibility of direct liver surface application as a drug targeting method. We employed

Changes in activity levels and isozyme patterns of isoleucine aminotransferase in response to experimentally induced hepatic lesion.

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Branched-chain amino acid aminotransferase activities were measured in rats in which hepatic lesions were induced experimentally, that is, fatty liver produced by an orotic acid-containing diet and acute hepatic lesion induced by D-galactosamine injection into the abdominal cavity. The levels of the

[Protective effect of prostaglandin E on hepatocytes and its value of early treatment of severe viral hepatitis].

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An animal model of hepatocytic necrosis was established with injection of D-galactosamine into peritoneal cavity. Examination at regular intervals after injection showed that the level of increased serum TB, ALT and GST and the degree of histological changes in the liver were less marked in

Effects of intestinal intervention on bacterial translocation in a rat model of acute liver failure in vivo.

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OBJECTIVE The study aimed to confirm bacterial translocation (BT) in rats with acute liver failure (ALF), to explore the correlation between the concentration of lipopolysaccharides and Toll-like receptor 4 (TLR4) expression, and further, to evaluate the curative effects of lactulose and

Glycoconjugate sugar residues in the chick embryo developing lung: a lectin histochemical study.

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A lectin histochemical study was performed to investigate the distribution and changes of the oligosaccharidic component of the glycoconjugates in the lung of chick embryos, of 1-day-old chick, and of the adult animal. For this purpose, a battery of seven horseradish peroxidase-conjugated lectins

Crystal structure of human GM2-activator protein with a novel beta-cup topology.

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GM2 activator protein (GM2-AP) belongs to a small group of non- enzymatic lysosomal proteins that act as cofactors in the sequential degradation of gangliosides. It has been postulated that GM2-AP extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A

Protective role of surface Toll-like receptor 9 expressing neutrophils in local inflammation during systemic inflammatory response syndrome in mice.

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Clinically, systemic inflammatory response syndrome (SIRS) occurs after serious trauma or sepsis. In sepsis, neutrophils are the major effector cells responsible for eliminating pathogens. However, the role of neutrophils in development of SIRS, especially in local inflammatory area, is

Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure.

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OBJECTIVE To investigate intraperitoneal transplantation of microencapsulated hepatic-like cells from human umbilical cord blood for treatment of hepatic failure in rats. METHODS CD34+ cells in umbilical cord blood cells were isolated by magnetic cell sorting. In the in vitro experiment, sorted

Cucurbit[7]uril: a high-affinity host for encapsulation of amino saccharides and supramolecular stabilization of their α-anomers in water.

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Cucurbit[7]uril (CB[7]), an uncharged and water-soluble macrocyclic host, binds protonated amino saccharides (D-glucosamine, D-galactosamine, D-mannosamine and 6-amino-6-deoxy-D-glucose) with excellent affinity (Ka =10(3) to 10(4) M(-1) ). The host-guest complexation was confirmed by NMR

[Lectin histochemical study on human dental pulp. Special reference to odontoblasts and pulp cells].

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The present study investigated some lectin affinities of human dental pulps, especially of odontoblasts and pulp cells. The materials were obtained from clinically intact teeth that were caries-free, attrition and/or abrasion-free. Mucopolysaccharide staining was carried out with applied PAS and

[The effect of tumor necrosis factor alpha on hepatic necrosis in viral hepatitis].

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In order to investigate the effect of tumor necrosis factor alpha (TNF alpha) on hepatocyte necrosis in viral hepatitis, TNF alpha with or without D-galactosamine (D-Gal) was injected into the abdominal cavity of rats. No effect was observed after injection of TNF alpha alone. After injection of TNF

[The effect of cytokines on the liver necrosis].

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OBJECTIVE To investigate the effect of cytokines on liver necrosis. METHODS We injected IL-1, IL-6, IFN gamma, TNF alpha with or without D-galactosamine (D-GAL) into the abdominal cavity of mice separately. RESULTS There was no effect on hepatocyte of normal mice after injection of IL-1, IL-6, IFN

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