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A defect in synapsis causes male sterility in a T-DNA-tagged Arabidopsis thaliana mutant.

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Fluorescence microscopy was used to study meiosis in microsporocytes from wild-type Arabidopsis thaliana and a T-DNA-tagged meiotic mutant. Techniques for visualizing chromosomes and beta-tubulin in other plant species were evaluated and modified in order to develop a method for analyzing meiosis in

An evolutionarily conserved mitochondrial orf108 is associated with cytoplasmic male sterility in different alloplasmic lines of Brassica juncea and induces male sterility in transgenic Arabidopsis thaliana.

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Nuclear-mitochondrial gene interactions governing cytoplasmic male sterility (CMS) in angiosperms have been found to be unique to each system. Fertility restoration of three diverse alloplasmic CMS lines of Brassica juncea by a line carrying the fertility-restorer gene introgressed from Moricandia

Close arrangement of CARK3 and PMEIL affects ABA-mediated pollen sterility in Arabidopsis thaliana

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ABA signaling is a vital plant signaling pathway for plant responses to stress conditions. ABA treatment can alter global gene expression patterns and cause significant phenotypic changes. We investigated the responses to ABA treatment during flowering in Arabidopsis thaliana. Dipping the flowers of

A cryptic cytoplasmic male sterility unveils a possible gynodioecious past for Arabidopsis thaliana.

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Gynodioecy, the coexistence of hermaphrodites and females (i.e. male-sterile plants) in natural plant populations, most often results from polymorphism at genetic loci involved in a particular interaction between the nuclear and cytoplasmic genetic compartments (cytonuclear epistasis): cytoplasmic

RNAi-mediated silencing of the Arabidopsis thaliana ULCS1 gene, encoding a WDR protein, results in cell wall modification impairment and plant infertility.

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Ubiquitin mediated protein degradation constitutes one of the most complex post translational gene regulation mechanisms in eukaryotes. This fine-tuned proteolytic machinery is based on a vast number of E3 ubiquitin ligase complexes that mark target proteins with ubiquitin. The specificity is

Disruption of Arabidopsis thaliana MYB26 results in male sterility due to non-dehiscent anthers.

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A male sterile mutant with a defect in anther dehiscence was identified in an Arabidopsis thaliana population mutagenized with the Zea mays transposon En-1/Spm. Mutants produce viable pollen that can fertilize when released mechanically from the anthers. Mutant stamens are of normal size and shape,

Disruption of the novel plant protein NEF1 affects lipid accumulation in the plastids of the tapetum and exine formation of pollen, resulting in male sterility in Arabidopsis thaliana.

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A novel male-sterile mutant of Arabidopsis thaliana was isolated by means of T-DNA tagging. Pollen abortion of the mutant was evident after microspore release, and pollen grains were completely absent at anthesis. Transmission electron microscope analysis revealed that primexine was coarsely

Temperature sensitive diphtheria toxin confers conditional male-sterility in Arabidopsis thaliana.

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A gene encoding a temperature-sensitive diphtheria toxin A chain (DTA) polypeptide was fused to the Arabidopsis thaliana tapetum-specific A9 promoter. Expression of the chimaeric gene in transgenic A. thaliana lines resulted in plants that were male-sterile, but female-fertile, when grown at 18

Male sterility associated with APRT deficiency in Arabidopsis thaliana results from a mutation in the gene APT1.

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Four mutants of Arabidopsis thaliana that are deficient in adenine phosphoribosyl transferase (APRT) activity have been isolated by selecting for germination of seeds and growth of the plantlets on a medium containing 2,6-diaminopurine (DAP), a toxic analog of adenine. In all mutants, DAP resistance

Double mutation of BRF1 and BRF2 leads to sterility in Arabidopsis thaliana.

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The TFIIB-related factor (BRF) family plays vital roles in RNA polymerase (Pol) III transcription initiation. However, little is known about the role of BRF in plants. Here, we report BRF1 and BRF2 are involved in Arabidopsis reproduction. In this study, we generated BRF1 and BRF2 double mutant

The simultaneous repression of CCR and CAD, two enzymes of the lignin biosynthetic pathway, results in sterility and dwarfism in Arabidopsis thaliana.

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Cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) catalyze the last steps of monolignol biosynthesis. In Arabidopsis, one CCR gene (CCR1, At1g15950) and two CAD genes (CAD C At3g19450 and CAD D At4g34230) are involved in this pathway. A triple cad c cad d ccr1 mutant, named ccc,

Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

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BACKGROUND The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main

[The expression profile of Arabidopsis thaliana β-1,3-glucanase promoter in tobacco].

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Well characterized promoters with specific activity only during male gametophyte development of transgenic organism are widely utilized in strategies aimed at the elimination of unwanted transgene escape from the transgenic to the non-transgenic plant population. Since the specificity and timing of

[Advanced biological studies on the cytoplasmic male sterility and its fertility restoration in plants].

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It was reviewed here that the major advances of biological research on the cytoplasmic male sterility (cms) and its fertility restoration in plants in the past decade. It focused on the identification of the cms- associated regions in the plant mitochondrial genome and their expressing profile, the

Disruption of the Arabidopsis RAD50 gene leads to plant sterility and MMS sensitivity.

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The Rad50 protein is involved in the cellular response to DNA-double strand breaks (DSBs), including the detection of damage, activation of cell-cycle checkpoints, and DSB repair via recombination. It is essential for meiosis in yeast, is involved in telomere maintenance, and is essential for

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