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Sivu 1 alkaen 97 tuloksia
Suberin deposition in potato periderm: a novel resistance mechanism against tuber greening.
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Light-induced tuber greening is one of the most important quality defects of potato. Although varietal and maturity factors are known to affect greening resistance, physiological mechanisms of resistance are poorly understood. We proposed that physiological and biochemical factors within the tuber
Effect of potato suberin on Streptomyces scabies proteome.
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Two-dimensional (2D) PAGE was used to detect proteins induced in Streptomyces scabies by potato suberin, a lipidic plant polymer. Nineteen up-regulated proteins were excised from 2D gels and analysed by N-terminal sequencing or tandem mass spectrometry (MS/MS). Four of the up-regulated proteins
[Enzyme activities and substrate levels of carbohydrate metabolism in proliferating and suberin synthesizing potato tuber cells].
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Isolation of tissue fragments from the potato tuber can initiate either periderm formation including suberin synthesis or cell proliferation without cicatrization effects. TCA-cycle activity has been shown to develop only in causal correlation with suberin synthesis (Lange, 1970). Biochemical
Comparative secretome analysis of Streptomyces scabiei during growth in the presence or absence of potato suberin.
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BACKGROUND
Suberin is a recalcitrant plant biopolymer composed of a polyphenolic and a polyaliphatic domain. Although suberin contributes to a significant portion of soil organic matter, the biological process of suberin degradation is poorly characterized. It has been suggested that Streptomyces
Proteome Analyses of Soil Bacteria Grown in the Presence of Potato Suberin, a Recalcitrant Biopolymer.
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Suberin is a complex lipidic plant polymer found in various tissues including the potato periderm. The biological degradation of suberin is attributed to fungi. Soil samples from a potato field were used to inoculate a culture medium containing suberin as the carbon source, and a metaproteomic
[Differentiated conditions for the induction of suberin synthesis or cell proliferation in potato tuber tissue after derepression].
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Experiments with potato tuber fragments under normal atmospheric conditions (0.03% CO2) suggest that a wound periderm, prominently marked by suberin synthesis, develops regularly, independently of preparation technics. It has been demonstrated that potato parenchym is able to perform differentiated
Structure, gas chromatographic measurement, and function of suberin synthesized by potato tuber tissue slices.
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The polymeric material (suberin) of the wound periderm of potato tuber slices was analyzed after depolymerization with LiAIH(4) in tetrahydrofuran or BF(3) in methanol with the use of thin layer chromatography, chemical modification, and combined gas-liquid chromatography and mass spectrometry.
Using trifluoroacetic acid to augment studies of potato suberin molecular structure.
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Systematically varied reaction times and concentrations of trifluoroacetic acid (TFA) have been used to remove polysaccharides associated with suberin isolated from potato wound periderm, thereby augmenting spectroscopic determinations of the molecular structure of this protective plant polyester.
The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids.
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The present study provides new insights on the role of the potato (Solanum tuberosum) suberin feruloyl transferase FHT in native and wound tissues, leading to conclusions about hitherto unknown properties of the phellogen. In agreement with the enzymatic role of FHT, it is shown that its
Partial depolymerization of genetically modified potato tuber periderm reveals intermolecular linkages in suberin polyester.
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Suberin is a biopolyester found in specialized plant tissues, both internal and external, with key frontier physiological functions. The information gathered so far from its monomer and oligomer composition, and in situ studies made by solid state techniques, haven't solved the enigma of how the
Physical, Chemical and Proteomic Evidence of Potato Suberin Degradation by the Plant Pathogenic Bacterium Streptomyces scabiei.
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Potato peels consist of a tissue called phellem, which is formed by suberized cell layers. The degradation of suberin, a lipidic and recalcitrant polymer, is an ecological process attributed to soil fungal populations; however, previous studies have suggested that Streptomyces scabiei, the causal
Potato suberin induces differentiation and secondary metabolism in the genus Streptomyces.
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Bacteria of the genus Streptomyces are soil microorganisms with a saprophytic life cycle. Previous studies have revealed that the phytopathogenic agent S. scabiei undergoes metabolic and morphological modifications in the presence of suberin, a complex plant polymer. This paper investigates
Chemical and ultrastructural evidence that waxes associated with the suberin polymer constitute the major diffusion barrier to water vapor in potato tuber (Solanum tuberosum L.).
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Combined gas chromatography-mass spectrometry showed that C21, C23, and C25 n-alkanes accumulated in the suberized layers during wound healing of cores of potato tuber tissue. Treatment (10 min) of freshly-cut tissue with trichloroacetate (TCA), an inhibitor of fatty-acid chain elongation, severely
Deposition of Aliphatic Suberin Monomers and Associated Alkanes during Aging of Solanum tuberosum L. Tuber Tissue at Different Temperatures.
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The effect of temperature on suberization of potato tuber tissue was measured by diffusive resistance and quantitative chemical procedures. The optimum temperature for formation of aliphatic suberin monomers and development of resistance to water vapor conduction was 26.4 degrees C whereas alkane
The Identification and Quantification of Suberin Monomers of Root and Tuber Periderm from Potato (Solanum tuberosum) as Fatty Acyl tert-Butyldimethylsilyl Derivatives.
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BACKGROUND
Protective plant lipophilic barriers such as suberin and cutin, with their associated waxes, are complex fatty acyl derived polyesters. Their precise chemical composition is valuable to understand the specific role of each compound to the physiological function of the
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