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xylose/karies

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ArtikkelitKliiniset tutkimuksetPatentit
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Tunable external cavity quantum cascade laser for the simultaneous determination of glucose and lactate in aqueous phase.

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A room temperature operated pulsed external-cavity (EC) quantum cascade laser (QCL) was used for mid-infrared (mid-IR) transmission measurements of glucose and lactate in aqueous solution. The high spectral power density of the EC-QCL (ranging from 1-350 mW) over a wide tuning range (1030-1230

Directed evolution of Thermotoga neapolitana xylose isomerase: high activity on glucose at low temperature and low pH.

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The Thermotoga neapolitana xylose isomerase (TNXI) is extremely thermostable and optimally active at 95 degrees C. Its derivative, TNXI Val185Thr (V185T), is the most active type II xylose isomerase reported, with a catalytic efficiency of 25.1 s(-1) mM(-1) toward glucose at 80 degrees C (pH 7.0).

Crystal structures of thermostable xylose isomerases from Thermus caldophilus and Thermus thermophilus: possible structural determinants of thermostability.

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The crystal structures of highly thermostable xylose isomerases from Thermus thermophilus (TthXI) and Thermus caldophilus (TcaXI), both with the optimum reaction temperature of 90 degrees C, have been determined by X-ray crystallography. The model of TcaXI has been refined to an R-factor of 17.8 %

Reptation-induced coalescence of tunnels and cavities in Escherichia Coli XylE transporter conformers accounts for facilitated diffusion.

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Structural changes and xylose docking to eight conformers of Escherichia Coli XylE, a xylose transporter similar to mammalian passive glucose transporters GLUTs, have been examined. Xylose docks to inward and outward facing conformers at a high affinity central site (K(i) 4-20 µM), previously

BACTEROIDES ORALIS, PROPOSED NEW SPECIES ISOLATED FROM THE ORAL CAVITY OF MAN.

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Loesche, W. J. (Forsyth Dental Center and Harvard School of Dental Medicine, Boston, Mass.), S. S. Socransky, and R. J. Gibbons. Bacteroides oralis, proposed new species isolated from the oral cavity of man. J. Bacteriol. 88:1329-1337. 1964.-Twenty-seven strains of oral bacteroides were

Co-factor binding confers substrate specificity to xylose reductase from Debaryomyces hansenii.

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Binding of substrates into the active site, often through complementarity of shapes and charges, is central to the specificity of an enzyme. In many cases, substrate binding induces conformational changes in the active site, promoting specific interactions between them. In contrast, non-substrates

First isolation of Candida dubliniensis from oral cavities of dermatological patients in Nanjing, China.

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Candida dubliniensis is an emerging pathogen capable of causing both superficial and systemic infections. Although C. dubliniensis and C. albicans are phenotypically similar, the two species differ in terms of epidemiology and the ability to rapidly develop resistance to fluconazole. C. dubliniensis

Systemic appearance of nutrients placed into the peritoneal cavity.

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The use of the peritoneal cavity as a route for delivery of nutrients in patients on continuous ambulatory peritoneal dialysis as well as patients not in renal failure has been proposed. In earlier studies we found dogs could be maintained for 30 days through continuous peritoneal infusion of a

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase.

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BACKGROUND Product inhibition can reduce catalytic performance of enzymes used for biofuel production. Different mechanisms can cause this inhibition and, in most cases, the use of classical enzymology approach is not sufficient to overcome this problem. Here we have used a semi-rational protein

Identification of eubacteria isolated from leaf cavities of four species of the N-fixing azolla fern as arthrobacter conn and dimmick.

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The nutritional and physiological characteristics of 15 isolates from four species of the Azolla fern were determined. Although some minor variation existed in levels of urease activity, ability to utilize xylose, and formation of N(2) gas from NO(3), all 15 isolates were rather similar and believed

Effect of temperature of incubation on performance of media in the detection of enteric pathogens.

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The effect of incubation temperatures on the efficacies of both plating media and transport or enrichment broths was determined by the analysis of 391 diarrheal stools for salmonellae and shigellae. Each analysis resulted in 90 observations. Stool specimens were homogenized in saline and used to

Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease.

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Actinobacillus actinomycetemcomitans from the human oral cavity was serologically characterized with rabbit antisera to the type strain NCTC 9710; a number of reference strains, including Y4, ATCC 29522, ATCC 29523, ATCC 29524, NCTC 9709; and our own isolates representative of each of 10 biotypes.

The in vitro propagation of stunting syndrome agent.

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Stunting syndrome (SS) is a viral enteric disease of turkey poults. The etiologic agent (stunting syndrome agent [SSA]) of this disease has been reported recently. The objective of this study was to develop a method for in vitro propagation of SSA. Primary cells, various continuous cell lines, and

Effect of temperature on transport and plating media for enteric pathogens.

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The effect of wide variations in incubation temperatures and long periods of incubation on transport and enrichment broths and plating media was determined by exhaustive analysis of 132 diarrheal stools for salmonellae and shigellae. Homogenized stools were streaked onto eosin methylene blue (EMB),
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