[Complete Freund's adjuvant-induced splenic T cell apoptosis in the prevention of diabetes in non-obese diabetic mice].
Mots clés
Abstrait
OBJECTIVE
To study the apoptosis of T lymphocytes in the spleen and its role in complete Freund's adjuvant (CFA) preventing diabetes in non-obese diabetic (NOD) mice.
METHODS
Forty-two NOD mice were divided into two groups randomly: CFA-treated NOD female mice (6w, 12w and 30w) and PBS-treated NOD female mice (6w, 12w and 30w). The apoptosis of splenic T lymphocytes was observed with the TDT-mediated fluorescein-dUTP nick-end labelling (TUNEL) technique and avidin-biotin complex method.
RESULTS
CFA induced the apoptosis of CD4+ T lymphocytes in the mouse spleen and could prevent the development of Type 1 diabetes (T1DM) in NOD female mice.
CONCLUSIONS
CFA preventing Type 1 diabetes in NOD mice may be related to the apoptosis of T lymphocytes in the spleen, which downregulates mature CD4+ T lymphocytes in the periphery.
