Preparation and application of molecularly imprinted polymer solid-phase microextraction fiber for the selective analysis of auxins in tobacco.

As signal molecules, auxins play an important role in mediating plant growth. Due to serious interfering substances in plants, it is difficult to accurately detect auxins with traditional solid-phase extraction methods. To improve the selectivity of sample pretreatment, a novel molecularly imprinted polymer -coated solid-phase microextraction fiber, which could be coupled directly to high-performance liquid chromatography, was prepared with indole acetic acid as template molecule for the selective extraction of auxins. The factors influencing the polymer formation, such as polymerization solvent, cross-linker, and polymerization time, were investigated in detail to enhance the performance of indole acetic acid-molecularly imprinted polymer coating. The morphological and chemical stability of this molecularly imprinted polymer-coated fiber was characterized by scanning electron microscopy, infrared spectrometry, and thermal analysis. The extraction capacity of the molecularly imprinted polymer-coated solid-phase microextraction fiber was evaluated for the selective extraction of indole acetic acid and indole-3-pyruvic acid followed by high-performance liquid chromatography analysis. The linear range for indole acetic acid and indole-3-pyruvic acid was 1-100 µg/L and their detection limit was 0.5 µg/L. The method was applied to the simultaneous determination of two auxins in two kinds of tobacco (Nicotiana tabacum L and Nicotiana rustica L) samples, with recoveries range from 82.1 to 120.6%.