Diterpenoids and Flavonoids from the Twigs of Cephalotaxus fortunei var. alpina.

Three new diterpenoids (a cephalotane, an abietane and a 9(10→20)-abeo-abietane) and one new flavonoid, together with 11 known compounds, were isolated from the twigs of Cephalotaxus fortunei var. alpina. The new compounds were identified by comprehensive spectroscopic (including 1D and 2D-NMR and HR-ESI-MS) analysis. Anti-inflammatory, immunosuppresive and cytotoxic activities of three new compounds (1, 7 and 15) were evaluated. Compound 7 showed weak cytotoxicity against tumor cell lines NCI-H1975, HepG2, MCF-7, while compounds 1 and 15 were not active at 80 μM. None of these compounds showed anti-inflammatory and immunosuppressive activities.