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amorphophallus coaetaneus/protease
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Genetic and biochemical characterization of a protease-resistant mesophilic β-mannanase from Streptomyces sp. S27.
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A β-mannanase gene, designated as man5S27, was cloned from Streptomyces sp. S27 using the colony polymerase chain reaction (PCR) method and expressed in Escherichia coli BL21 (DE3). The open reading frame consisted of 1,161 bp and encoded a 386-amino-acid polypeptide (Man5S27) with calculated
Effect of prebiotic konjac mannanoligosaccharide on growth performances, intestinal microflora, and digestive enzyme activities in yellow catfish, Pelteobagrus fulvidraco.
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In the present study, konjac mannanoligosaccharide (KMOS) was evaluated as a prebiotic in yellow catfish. The fish were fed with diets containing KMOS in four concentrations: 0 g kg(-1) (C), 1.0 g kg(-1) (KM1), 2.0 g kg(-1) (KM2), and 3.0 g kg(-1) (KM3) for 49 days, respectively. Another group fed
Effect of indigestible polysaccharides on pancreatic exocrine secretion and biliary output.
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The present study was designed to determine the effect of purified viscous polysaccharides on pancreatic exocrine secretion and biliary output. Male Sprague-Dawley rats aged 5 weeks were fed either on the control diet which contained no fiber or on the experimental diets that contained 5%
A Novel endo-1,4-β-mannanase from Bispora antennata with good adaptation and stability over a broad pH range.
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An endo-β-1,4-mannanase encoding gene, man5, was cloned from Bispora antennata CBS 126.38, which was isolated from a beech stump. The cDNA of man5 consists of 1,299 base pairs and encodes a 432-amino-acid protein with a theoretical molecular mass of 46.6 kDa. Deduced MAN5 exhibited the highest amino
Acidic β-mannanase from Penicillium pinophilum C1: Cloning, characterization and assessment of its potential for animal feed application.
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The β-mannanase gene, man5C1, was cloned from Penicillium pinophilum C1, a strain isolated from the acidic wastewater of a tin mine in Yunnan, China, and expressed in Pichia pastoris. The sequence analysis displayed the gene consists of a 1221-bp open reading frame encoding a protein of 406 amino
Bowel microbiota moderate host physiological responses to dietary konjac in weanling rats.
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Diets rich in complex carbohydrates that resist digestion in the small bowel can alter large bowel ecology and microbiota biochemistry because the carbohydrates become substrates for bacterial growth and metabolism. Conventional or germ-free weanling rats were fed a control diet or diets containing
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