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Biotin-responsive in vivo carboxylase deficiency in two siblings with secretory diarrhea receiving total parenteral nutrition.
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Two siblings with a congenital syndrome of secretory diarrhea and seizures developed progressive skin rash, alopecia, and mucocutaneous candidiasis while receiving biotin-free total parenteral nutrition. Abnormally low urinary biotin excretion was associated with these clinical findings, but the
Practical and economical method for using biotinylated DNA probes with bacterial colony blots to identify diarrhea-causing Escherichia coli.
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A simple and economical method was developed for using biotinylated DNA probes to hybridize with bacterial colonies belonging to the various categories of diarrhea-causing Escherichia coli. Simplification and cost containment were achieved by using Whatman no. 541 filter papers instead of
Detection of cytopathic and noncytopathic bovine viral diarrhea virus in cell culture with an immunoperoxidase test.
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Bovine viral diarrhea virus (BVDV) antigen was detected in cell culture with an indirect immunoperoxidase (IP) procedure using a specific monoclonal antibody, and an avidin-biotin-peroxidase complex. Cytopathic and noncytopathic strains of the virus showed similar patterns of IP staining until 3
Phase II trial of yttrium-90-DOTA-biotin pretargeted by NR-LU-10 antibody/streptavidin in patients with metastatic colon cancer.
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A Phase II study of yttrium-90-tetra-azacyclododecanetetra-acetic acid-biotin (90Y-DOTA-biotin) pretargeted by NR-LU-10 antibody/streptavidin (SA) was performed. The primary objectives of the study were to evaluate the efficacy and safety of this therapy in patients with metastatic colon cancer.
Antiviral Compounds Against Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus.
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Porcine epidemic diarrhea (PED) is a severe diarrhea disease in swine that is caused by porcine epidemic diarrhea virus (PEDV). Nucleocapsid (N) protein is the RNA-binding protein of PEDV, which plays an important role for virus life cycle. The aim of this research was to screen and characterize the
Enzyme-linked immunosorbent assay assessment of bovine viral diarrhea virus antigen in inactivated vaccines using polyclonal or monoclonal antibodies.
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An enzyme-linked immunosorbent assay (ELISA) procedure was developed to assay the cytopathic and noncytopathic bovine viral diarrhea (BVD) virus strains used in inactivated vaccines licensed by the United States Department of Agriculture. The assay uses a biotin-labeled, staphylococcal protein A
Competitive and blocking enzyme-linked immunoassay for detection of fetal bovine serum antibodies to bovine viral diarrhea virus.
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A competitive blocking enzyme-linked immunoassay (CELIA) was developed to detect bovine viral diarrhea virus (BVDV) antibodies in undiluted fetal bovine serum (FBS). The CELIA was based on competition of serum BVDV antibodies with biotin-labelled anti-BVDV immunoglobulins (Ig) for a limited quantity
Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR.
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To establish a practical method for detecting porcine epidemic diarrhea virus (PEDV), the use of primers derived from sequences that amplify the M protein genes of PEDV in a RT-PCR detection system was investigated. Primers were designed to amplify a 854-bp fragment by RT-PCR. This reaction was
Application of the biotin-labeled toxin mutant for affinity isolation of associated proteins in the mammalian cells.
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Cholera toxin (CT), one of the AB5 bacterial toxin families, is produced by Vibrio cholerae, breeches the intestinal epithelial barrier and enters host epithelial cells to cause the massive secretory diarrhea. This study focused on understanding the retro-translocation machinery of the bacterial
Salmonella infection inhibits intestinal biotin transport: cellular and molecular mechanisms.
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Infection with the nontyphoidal Salmonella is a common cause of food-borne disease that leads to acute gastroenteritis/diarrhea. Severe/prolonged cases of Salmonella infection could also impact host nutritional status, but little is known about its effect on intestinal absorption of vitamins,
Radiation absorbed dose estimation for 90Y-DOTA-biotin with pretargeted NR-LU-10/streptavidin.
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Pretargeted radioimmunotherapy permits the administration of doses of 90Y five times higher than is possible with antibodies directly labeled with 90Yttrium (90Y). These high doses of 90Y introduced new issues for dosimetry that were not encountered in prior studies using conventional
Morphologic changes and immunohistochemical detection of viral antigen in ovaries from cattle persistently infected with bovine viral diarrhea virus.
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OBJECTIVE
To identify morphologic differences in ovaries from cows persistently infected with bovine viral diarrhea virus (BVDV) and determine ovarian cell types infected in these cows.
METHODS
A comparative study of ovaries in cattle persistently infected with BVDV and cattle not persistently
Immunoperoxidase procedures for rapid detection of bovine viral diarrhea-mucosal disease virus antigen.
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Bovine viral diarrhea-mucosal disease (BVD-MD) virus antigen was detected in bovine fetal muscular cells from sera of 42 cattle persistently infected with noncytopathic BVD-MD virus by the indirect immunoperoxidase procedure (IIP) but not from sera of 100 apparently healthy cattle. These findings
The fecal microbiome in cats with diarrhea.
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Recent studies have revealed that microbes play an important role in the pathogenesis of gastrointestinal (GI) diseases in various animal species, but only limited data is available about the microbiome in cats with GI disease. The aim of this study was to evaluate the fecal microbiome in cats with
Epidemiological survey of Blastocystis hominis in Huainan City, Anhui Province, China.
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OBJECTIVE
To provide scientific evidence for prevention and controlling of blastocystosis, the infection of Blastocystis homonis and to study its clinical significance in Huainan City, Anhui Province, China.
METHODS
Blastocystis homonis in fresh stools taken from 100 infants, 100 pupils, 100 middle
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