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brassica campestris/l tyrosine
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Double-edged sword effect of biochanin to inhibit nuclear factor kappaB: suppression of serine/threonine and tyrosine kinases.
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Several protein tyrosine kinase (PTK) inhibitors predominantly isoflavones, such as genistein, erbstatin, quercetin, daidzein, present in red clover, cabbage and alfalfa, show apoptotic effect against cancer cells. In this study I found that biochanin, a methoxy form of genistein, inhibits
A simple and sensitive fluorescent sensor for methyl parathion based on L-tyrosine methyl ester functionalized carbon dots.
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In this paper, a simple and sensitive fluorescent sensor for methyl parathion is developed based on L-tyrosine methyl ester functionalized carbon dots (Tyr-CDs) and tyrosinase system. The carbon dots are obtained by simple hydrothermal reaction using citric acid as carbon resource and L-tyrosine
The intensity of tyrosine nitration is associated with selenite and selenate toxicity in Brassica juncea L.
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Selenium phytotoxicity involves processes like reactive nitrogen species overproduction and nitrosative protein modifications. This study evaluates the toxicity of two selenium forms (selenite and selenate at 0µM, 20µM, 50µM and 100µM concentrations) and its correlation with protein tyrosine
Reorganization of Protein Tyrosine Nitration Pattern Indicates the Relative Tolerance of Brassica napus (L.) over Helianthus annuus (L.) to Combined Heavy Metal Treatment
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Metal-polluted areas, especially where municipal sewage is used as fertilizer, often have high concentrations of more than one metal. The development of the root system is regulated by a complex signaling network, which includes reactive oxygen and nitrogen species. The delicate balance of the
Characterization of a novel gene, BcMF7, that is expressed preferentially in pollen of Brassica campestris L. ssp. chinensis Makino.
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Pollen formation is important for plant sexual reproduction. To identify the genes that are involved in pollen formation, we performed the genome-wide transcriptional profiling in the flower buds of both male meiotic cytokinesis (mmc) mutant and its wild-type plants of Brassica campestris L. ssp.
Production of Brassica carinata protein hydrolyzates with a high Fischer's ratio using immobilized proteases.
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Brassica carinata protein isolates were hydrolyzed using the digestive enzymes trypsin, chymotrypsin, and carboxypeptidase A in order to obtain hydrolyzates with a high Fischer's ratio. The proteases were immobilized using two glyoxyl-agarose supports of different porosity, 4 and 10% agarose gels,
Genomic and Post-Translational Modification Analysis of Leucine-Rich-Repeat Receptor-Like Kinases in Brassica rapa.
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Among several receptor-like kinases (RLKs), leucine-rich-repeat receptor-like kinases (LRR-RLKs) are a major group of genes that play crucial roles in growth, development and stress responses in plant systems. Given that they have several functional roles, it is important to investigate their roles
Growth conditions, elemental accumulation and induced physiological changes in Chinese cabbage.
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Soils contaminated with low levels of heavy metals and other trace elements are now frequently used for vegetable growing. In this situation, heavy metals and trace elements from these polluted soils may accumulate in the agricultural plants being grown in them and thereby enter the human food
Similarities and differences of five peroxidases from turnip and horseradish. Peptide mapping studies on glycoproteins.
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Four isoperoxidases of turnip root and isoperoxidase C of horseradish root were digested with trypsin, and their peptide maps, prepared by high-voltage paper electrophoresis, were compared. All five tryptic digests were completely soluble at pH 8. The maps were developed with a variety of general
Purification and Characterization of a Potato Tuber Acid Phosphatase Having Significant Phosphotyrosine Phosphatase Activity.
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The major acid phosphatase (APase) from potato (Solanum tuberosom L. cv Chiefton) tubers has been purified 2289-fold to near homogeneity and a final O-phospho-L-tyrosine (P-Tyr) hydrolyzing specific activity of 1917 [mu]mol Pi produced min-1 mg-1 of protein. Nondenaturing polyacrylamide gel
A new member of plant CS-lyases. A cystine lyase from Arabidopsis thaliana.
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Cystine lyases catalyze the breakdown of l-cystine to thiocysteine, pyruvate, and ammonia. Until now there are no reports of the identification of a plant cystine lyase at a molecular level, and it is not clear what biological role this class of enzymes have in plants. A cystine lyase was isolated
Single-step purification of peroxidase by 4-aminobenzohydrazide from Turkish blackradish and Turnip roots.
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Peroxidases (PODs) were purified from the Turkish blackradish (Raphanus sativus L.) (TBR) and Turnip (Brassica rapa L.) using a simple and effective single-step method. An affinity resin was synthesised by coupling the 4-aminobenzohydrazide ligand and the l-tyrosine spacer-arm to
Increased viral yield and symptom severity result from a single amino acid substitution in the turnip yellow mosaic virus movement protein.
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Turnip yellow mosaic virus is a positive-strand RNA virus that produces light green or yellow-green mosaic symptoms in Chinese cabbage plants. We have characterized a strain that produces nearly uniform yellow-green chlorosis in systemically infected Chinese cabbage leaves. The increased symptom
The turnip yellow mosaic virus tRNA-like structure cannot be replaced by generic tRNA-like elements or by heterologous 3' untranslated regions known to enhance mRNA expression and stability.
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The tRNA-like structure (TLS) at the 3' end of the turnip yellow mosaic virus genome was replaced with heterologous tRNA-like elements, and with a poly(A) tail, in order to assess its role. Replacement with the valylatable TLSs from two closely related tymoviruses resulted in infectious viruses. In
Studies of virus structure by laser-Raman spectroscopy. Turnip yellow mosaic virus and capsids.
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Laser-Raman spectroscopy of the turnip yellow mosaic virus (TYMV) and its capsid indicate the following features of the structure and assembly of the virion. The secondary structure of coat-protein molecules in TYMV is comprised of 9 +/- 5% alpha-helix, 43 +/- 6% beta-sheet, and 48 +/- 6% irregular
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