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carob tree/− nicotine
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Formation of protoplasts from cultured tobacco cells and Arabidopsis thaliana by the action of cellulosomes and pectate lyase from Clostridium cellulovorans.
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The crude culture supernatants from Clostridium cellulovorans were tested for their ability to convert plant cells to protoplasts. The supernatants readily released protoplasts from cultured tobacco cells and Arabidopsis thaliana. The crude culture supernatant from pectin-grown cells was more active
Adhesion of polysaccharides to intact cells and protoplasts of Nicotiana tabacum BY-2 and its stimulative effects on protoplast growth.
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The adhesion of water-soluble beta-d-glycans, including cellulose-adhesive schizophyllan, xyloglucan, and locust bean gum to intact cells and protoplasts of Nicotiana tabacum BY-2 was examined using their fluorescent derivatives. Fluorescence microscopy showed that schizophyllan, xyloglucan, locust
Mannan oligosaccharides trigger multiple defence responses in rice and tobacco as a novel danger-associated molecular pattern.
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Oligosaccharide, a typical danger-associated molecular pattern (DAMP), has been studied and applied as plant defence elicitor for several years. Here, we report a novel oligosaccharide, mannan oligosaccharide (MOS) with a degree of polymerization of 2-6, which was hydrolysed from locust bean gum by
Purification and characterization of a cell-surface lectin (Lectin II) from Agrobacterium radiobacter NCIM 2443.
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A lectin was isolated from Agrobacterium radiobacter cell surface and purified. It is a monomer of 40 kDa as shown by SDS-PAGE. The lectin has a pI of 9.15 and amino acid composition of the lectin shows that 44% of the amino acids are hydrophobic. The lectin agglutinates rabbit erythrocytes but does
Development of a quantitative method for determination of the optimal conditions for protoplast isolation from cultured plant cells.
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An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the
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