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The genome of simian hemorrhagic fever virus.
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Techniques are described for preparing intact Simian Hemorrhagic Fever (SHF) virus RNA. SHF RNA extracted by proteinase K digestion in the presence of sodium dodecyl sulphate (SDS) has a sedimentation coefficient of 49 S compared with a reference figure of 47 S for Sindbis RNA. Purified SHF RNA in
Studies on simian hemorrhagic fever virus nucleocapsids.
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Purified Simian Hemorrhagic Fever (SHF) virions are shown to have a sedimentation coefficient of 214S when compared with previously determined figures for Sindbis virus cosedimented in neutral sucrose gradients. The nucleic acid content in SHF virions was about 8 per cent of the total virionic mass.
Hyperthermia Induced by Near-Infrared Laser-Irradiated CsWO3 Nanoparticles Disintegrates Preformed Lysozyme Amyloid Fibrils.
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This research study attempts to prove the concept of the applicability of hyperthermia to treating the lysozyme amyloid fibrils (LAF)'s self-assembled fibrillary aggregates by a feedback-modulated temperature controller ranging from 26 °C to 80 °C, and separately, by near-infrared (NIR)
Characterization of Hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome.
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Hantaan virus, strain 76-118, was propagated to high titer in a clone of Vero cells, and infectious virions were successfully concentrated and purified. Infectivity and virus antigenic activity were closely associated with a virus particle that exhibited a sedimentation rate indistinguishable from a
Magneto-Fluorescent Perovskite Nanocomposites for Directed Cell Motion and Imaging.
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The ability for a magnetic field to penetrate biological tissues without attenuation has led to significant interest in the use of magnetic nanoparticles for biomedical applications. In particular, active research is ongoing in the areas of magnetically guided drug delivery and magnetic hyperthermia
Isolation and characterization of a plasmid from phase I Coxiella burnetii.
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The DNA from the Nine Mile phase I strain of Coxiella burnetti, the etiological agent of Q fever, has been isolated and purified by cesium chloride-ethidium bromide density gradient centrifugation. A fraction of this DNA has a density characteristic of plasmid DNA. The plasmid DNA was cut with 20
Detection of hantavirus RNA in tissues of experimentally infected mice using reverse transcriptase-directed polymerase chain reaction.
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Detection of hantaviruses, the etiological agents of hemorrhagic fever with renal syndrome (HFRS), by virus isolation using experimental animals or cell culture is time-consuming. A more rapid but equally specific method is needed. We used a reverse transcriptase-directed polymerase chain reaction
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