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chlamydia infections/potassium
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Treatment of cervical chlamydial infection with amoxicillin/clavulanate potassium.
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OBJECTIVE
To determine if amoxicillin/clavulanate potassium is effective in the treatment of Chlamydia trachomatis endocervicitis.
METHODS
Thirty-two patients with culture-proven endocervical infection were treated with amoxicillin/clavulanate potassium, 500 mg orally 3 times a day for 10 days.
Structural analysis of the lipopolysaccharide from Chlamydia trachomatis serotype L2.
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The lipopolysaccharide (LPS) of Chlamydia trachomatis L2 was isolated from tissue culture-grown elementary bodies using a modified phenol/water procedure followed by extraction with phenol/chloroform/light petroleum. From a total of 5 x 10(4) cm2 of infected monolayers, 22.3 mg of LPS were obtained.
Loss of inorganic ions from host cells infected with Chlamydia psittaci.
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Mouse fibroblasts (L cells) infected with the 6BC strain of Chlamydia psittaci released potassium ion (K(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. When the multiplicity of infection was 500 to 1,000 50% infectious units (ID(50)) per L
Purification of Chlamydia psittaci antigen by affinity chromatography on polymyxin B agarose for use in the enzyme-linked immunosorbent assay (ELISA).
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The purification of cell wall antigens of Chlamydia psittaci by affinity chromatography on polymyxin B agarose is described. Chlamydial cell wall antigens were prepared using different methods: heat treatment, ultrasonication and sodium deoxycholate treatment. The antigens were subsequently purified
Preparation of complement fixation antigen of Chlamydia psittaci grown in tissue culture by treatment with beta-propiolactone.
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A new method of preparing a chlamydial complement fixation (CF) antigen by treatment with beta-propiolactone (BPL) is presented. Chlamydia psittaci strains Pigeon-1041 and Budgerigar-No. 1, and Chlamydia trachomatis strain L2/434/BU, propagated in L-929 cell monolayers, were inactivated with BPL.
Synthesis of protein in host-free reticulate bodies of Chlamydia psittaci and Chlamydia trachomatis.
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Synthesis of protein by the obligate intracellular parasitic bacteria Chlamydia psittaci (6BC) and Chlamydia trachomatis (serovar L2) isolated from host cells (host-free chlamydiae) was demonstrated for the first time. Incorporation of [35S]methionine and [35S]cysteine into trichloroacetic
Chlamydia protein Pgp3 studied at high resolution in a new crystal form.
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The protein Pgp3 is implicated in the sexually transmitted disease chlamydia and comprises an extended complex arrangement of a C-terminal domain (CTD) and an N-terminal domain (NTD) linked by a triple-helix coiled coil (THCC). Here, the X-ray crystal structure of Pgp3 from an LGV1 strain is
Chlamydial infection of monocytes stimulates IL-1beta secretion through activation of the NLRP3 inflammasome.
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Chlamydia trachomatis infections represent the leading cause of bacterial sexually-transmitted disease in the United States and can cause serious tissue damage leading to infertility and ectopic pregnancies in women. Inflammation and hence the innate immune response to chlamydial infection
Inflammation and fibrosis during Chlamydia pneumoniae infection is regulated by IL-1 and the NLRP3/ASC inflammasome.
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Chlamydia pneumoniae is a common respiratory pathogen associated with atypical pneumonia, and it has been suggested as a trigger or promoter of several chronic inflammatory conditions, such as asthma and atherosclerosis. The beta form of IL-1 (IL-1beta) is a proinflammatory cytokine released by many
Critical role for interleukin-1beta (IL-1beta) during Chlamydia muridarum genital infection and bacterial replication-independent secretion of IL-1beta in mouse macrophages.
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Recent findings have implicated interleukin-1beta (IL-1beta) as an important mediator of the inflammatory response in the female genital tract during chlamydial infection. But how IL-1beta is produced and its specific role in infection and pathology are unclear. Therefore, our goal was to determine
Electron Microscopic Observations on the Fine Structure of Cell Walls of Chlamydia psittaci.
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L-cell cultures were infected with elementary bodies (EB) of meningopneumonitis organisms. Cell walls were prepared from reticulate bodies (RB), which are the intracellular developmental forms into which EB are converted, and from EB at appropriate times after infection. When fragmented EB cell
Value of wet mount and cervical cultures at the time of cervical cytology in asymptomatic women.
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OBJECTIVE
To correlate Papanicolaou smear findings with the wet mount and cervical culture results in asymptomatic patients, and to review the value of doing wet mount and/or cervical cultures in these patients at the time of Papanicolaou smear.
METHODS
Asymptomatic women presenting for routine
Ulcus vulvae acutum in a 13-year-old girl after influenza A infection.
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A 13-year-old otherwise healthy premenarchal girl presented with acute onset of painful vulvar ulcerations. One day before developing vulvar ulcerations, she experienced flu-like symptoms, including a low-grade fever, cough, sore throat, and myalgia. Results of a throat swab were positive for
"Shotgun" versus sequential testing. Cost-effectiveness of diagnostic strategies for vaginitis.
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BACKGROUND
Although vaginitis is a common outpatient problem, only 60% of patients can be diagnosed at the initial office visit of a primary care provider using the office procedures of pH testing, whiff tests, normal saline, and potassium hydroxide preps.
OBJECTIVE
To determine the most
Comparison between vaginal swab and endocervical swab during pelvic examination.
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OBJECTIVE
While wet mounts and potassium hydroxide (KOH) preparations are frequently used to help diagnose vaginitis and cervicitis, the sample site varies. This study was conducted to evaluate whether the specimen site (vaginal pool versus endocervical specimen) affects diagnostic
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