3 résultats
A variant clone of Hyoscyamus muticus (VIIIB9) with a specific, stable requirement for tryptophan has been shown to have the following characteristics: (i) no accumulation of tryptophan from anthranilic acid; (ii) growth on added tryptophan or indole but not on anthranilic acid; (iii) accumulation
The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two
Complementation of two metabolic deficiences - nitrate reductase and tryptophan synthase - was used to select for somatic fusion hybrids between tobacco (Nicotiana tabacum) and henbane (Hyoscyamus muticus) with prior X-irradiation of one partner. Using species specific, radioactively labelled DNA