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hyoscyamus/scopolamine
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Phytochemical investigation of Hyoscyamus albus.
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The work presented in this paper illustrates the isolation and structure elucidation of secondary metabolites of Hyoscyamus albus. Two new natural source and three known compounds were isolated from the Hyoscyamus albus. Among the isolated compounds, grivilloside H (1) and betulaplatoside (2) were
Metabolic Characterization of Hyoscyamus niger Ornithine Decarboxylase.
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Ornithine decarboxylase (ODC) catalyzes ornithine decarboxylation to yield putrescine, a key precursor of polyamines, and tropane alkaloids (TAs). Here, to investigate in depth the role of ODC in polyamine/TA biosynthesis and to provide a candidate gene for engineering polyamine/TA production, the
Scopolamine production in suspension cultures and redifferentiated roots of Hyoscyamus niger.
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Adventitious roots were induced from 2-year-old, hyoscyamine rich suspension cultures of Hyoscyamus niger L. The cultured roots grew well in Linsmaier-Skoog medium containing 10 (-8)M 6-benzyladenine with 3% sucrose and produced much more scopolamine (0.12-0.30% dW) than hyoscyamine (0.04-0.08% dW).
[Henbane (Hyoscyamus reticulatus) poisoning in children in the Negev].
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During 1984-1989, 19 Bedouin children, 4-8 years old, were hospitalized because of henbane plant (Hyoscyamus reticulatus) poisoning. There were 14 cases in the autumn, 3 in the spring and 2 in the summer. The most prominent signs were altered state of consciousness (including deep coma in 3) and
Production of tropane alkaloids in cultured cells of Hyoscyamus niger.
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Tests for calluses rich in tropane alkaloids were made with newly induced calluses of Atropa belladonna, Datura stramonium and Hyoscyamus niger. Only calluses of H. niger gave an alkaloid-positive test.A Hyoscyamus cell line had the highest total alkaloid content of all the calluses screened by the
Optimization of some Parameters in Cultivating Scopolamine-producing Cells of Hyoscyamus muticus.
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Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.
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Scopolamine is a pharmaceutically important tropane alkaloid extensively used as an anticholinergic agent. Here, we report the simultaneous introduction and overexpression of genes encoding the rate-limiting upstream enzyme putrescine N-methyltransferase (PMT) and the downstream enzyme hyoscyamine 6
Promoting the Accumulation of Scopolamine and Hyoscyamine in Hyoscyamus Niger L. Through EMS Based Mutagenesis
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The overexploitation of medicinal plants is depleting gene pool at an alarming rate. In this scenario inducing the genetic variability through targeted mutations could be beneficial in generating varieties with increased content of active compounds. The present study aimed to develop a reproducible
Somaclonal variation in transformed roots and protoplast-derived hairy root clones of Hyoscyamus muticus.
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Substantial somaclonal variation in growth rate, morphology, and alkaloid production of Hyoscyamus muticus L. hairy root clones obtained by transformation with four Agrobacterium strains was shown. The hyoscyamine content of the root clones (n = 100) obtained from the same origin varied from 0.03 to
Variation in the Tropane Alkaloid Content of Hyoscyamus muticus Plants and Cell Culture Clones.
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Systematic studies were carried out on two different strains (Gatersleben and Cairo) of HYOSCYAMUS MUTICUS L. (Solananaceae) in order to analyse the variation in the contents of the two main tropa-alkaloids in individual plants and protoplast-derived cell culture clones. The hyoscyamine content was
Insecticidal Activity of Hyoscyamus niger L. on Lucilia sericata Causing Myiasis
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Background: Hyoscyamus niger L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the
Determination of the main tropane alkaloids from transformed Hyoscyamus muticus plants by capillary zone electrophoresis.
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A capillary zone electrophoretic method (CZE) was developed using an uncoated fused silica capillary for the separation and determination of the main tropane alkaloids. The applicability of the developed method for analysis of plant samples was examined by analyzing samples of transgenic Egyptian
Responses of fungi to tropane alkaloids produced by a medicinal plant Hyoscyamus muticus (Egyptian henbane).
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Antifungal activity of hyoscyamine (Hcy) and scopolamine (Sco) were determined by TLC-bioautography against fungi associated with H. muticus grown in Egypt, and those isolated from other plants grown in Japan. All 40 fungal strains were tolerant to Sco and sensitive to Hcy, exhibiting a growth
Inorganic ions in the medium modify tropane alkaloids and riboflavin output in Hyoscyamus niger root cultures.
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BACKGROUND
Hyoscyamus niger L. (Solanaceae) roots are rich of tropane alkaloids, such as hyoscyamine and scopolamine are used as the source of raw material for the pharmaceutical industry.
OBJECTIVE
The aim of the present study was to investigate the effect of calcium, magnesium, and iron ions on
An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis.
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A cDNA encoding hyoscyamine 6β-hydroxylase (H6H, EC 1.14.11.11), a bifunctional enzyme catalyzing the last two steps in the scopolamine biosynthetic pathway, was isolated from Hyoscyamus senecionis, a medicinal plant endemic to the Iranian plateau. Expression analysis indicates that Hsh6h is
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