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Direct inhibitory effect of rotavirus NSP4(114-135) peptide on the Na(+)-D-glucose symporter of rabbit intestinal brush border membrane.
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The direct effect of a rotavirus nonstructural glycoprotein, NSP4, and certain related peptides on the sodium-coupled transport of D-glucose and of L-leucine was studied by using intestinal brush border membrane vesicles isolated from young rabbits. Kinetic analyses revealed that the NSP4(114-135)
Rotavirus infection impairs intestinal brush-border membrane Na(+)-solute cotransport activities in young rabbits.
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The mechanism of rotavirus diarrhea was investigated by infecting young, specific pathogen-free, New Zealand rabbits with a lapine rotavirus, strain La/RR510. With 4-wk-old animals, virus shedding into the intestinal lumen peaked at 72 h postinfection (hpi), and a mild, watery diarrhea appeared at
Amelioration of methotrexate-induced malabsorption by vitamin A.
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Methotrexate (MTX) induces damage to the small intestine, resulting in malabsorption and diarrhea. We found that the coadministration of vitamin A (VA) with MTX protected the small intestine from MTX-induced damage. In this study, the permeability of D-glucose, D-xylose and L-leucine through the
Effect of folate deficiency of the intestinal mucosa on jejunal transport in the rat.
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The role of folate deficiency in the pathogenesis of the abnormalities of structure and transport of the small intestine that are often present in certain disorders such as alcoholism is unclear. One reason for this is that the folate status of the intestinal mucosa has never been ascertained in
Effect of mucosal amino acids on SCC and Na and Cl fluxes in the porcine small intestine.
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Most amino acids are, like glucose, co-transported with sodium and are thereby potential additives to oral rehydration solutions for the treatment of diarrhea. In this study the effects of mucosal amino acids on short-circuit current and Na and Cl fluxes in three segments of the porcine small
Diagnosis of congenital glucose-galactose malabsorption by measuring sugar- and amino acid-evoked potential differences in jejunum in vivo.
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A girl who was clinically suspected of having congenital glucose-galactose malabsorption was investigated for sugar- and amino acid-evoked potential differences in the jejunum in vivo using a jejunal perfusion technique. She was fed an ordinary diet without suffering diarrhea at the time (at age 2)
Dietary Leucine Supplementation Improves the Mucin Production in the Jejunal Mucosa of the Weaned Pigs Challenged by Porcine Rotavirus.
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The present study was mainly conducted to determine whether dietary leucine supplementation could attenuate the decrease of the mucin production in the jejunal mucosa of weaned pigs infected by porcine rotavirus (PRV). A total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1
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