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peroxidase/cannabis
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Cytotoxic Effects of Cannabinoids on Human HT-29 Colorectal Adenocarcinoma Cells: Different Mechanisms of THC, CBD, and CB83
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In this study, we investigated the effects of exposition to IC50 dose for 24 h of a new synthetic cannabinoid (CB83) and of phytocannabinoids Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) on HT-29 colorectal carcinoma cells. Cell viability and proliferative activity evaluated using
An immunohistochemical study of the thyroid parafollicular (C) cells in rats treated with cannabinoids - preliminary investigations.
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The purpose of the present study was to evaluate the effect of a single intraperitoneal injection of a stable analogue of endogenous cannabinoid anandamide - R-(+)-methanandamide (2.5 mg/kg) and CP 55,940 (0.25 mg/kg), an egzogenous CB1 receptor-agonist, on the calcitonin (CT) immunoreactivity of
The debate about marijuana usage in transplant candidates: recent medical evidence on marijuana health effects.
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OBJECTIVE
Cannabis is currently the most widely used illicit substance in the world. The issue of how to handle transplant candidates with active use of cannabis is a commonly encountered one for transplant-selection committees.
RESULTS
Correlates of marijuana use include increased risk of use of
Enhanced chemiluminescent enzyme immunoassay for cannabinoids in urine.
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An enhanced chemiluminescent enzyme immunoassay was developed for the detection of cannabinoids in urine. It utilises an antiserum specific for tetrahydrocannabinol and its major metabolite, a donkey anti-sheep antiserum and a horseradish peroxidase labelled antigen conjugate. The bound enzyme is
Cannabinoid-deficient Benin republic hemp (Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status.
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Localization of the CB1 type cannabinoid receptor in the rat basolateral amygdala: high concentrations in a subpopulation of cholecystokinin-containing interneurons.
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The neuronal localization of the CB1 cannabinoid receptor in the rat basolateral amygdala was studied using peroxidase and fluorescence immunohistochemical techniques. All nuclei of the basolateral amygdala contained a large number of lightly stained pyramidal neurons and a small number of more
Localization of cannabinoid CB1 receptors in the human anterior eye and retina.
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OBJECTIVE
To determine the presence and distribution of CB1 cannabinoid receptors within the human eye.
METHODS
A subtype-specific affinity-purified polyclonal antibody to the cannabinoid CB1 receptor was used to determine CB1 localization. Postmortem human eyes were fixed in methacarn and embedded
Effect of Hempseed (Cannabis sativa sp.) Inclusion to the Diet on Performance, Carcass and Antioxidative Activity in Japanese Quail (Coturnix coturnix japonica).
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This study was conducted to determine the effects of hempseed (H) on performance, carcass traits, and antioxidant activity in Japanese quail (Coturnix coturnix japonica). A total of 192 quail with seven-days old were divided into four experimental groups with four replicates. The treatments were; i)
Biochemical differences in Cannabis sativa L. depending on sexual phenotype.
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Hemp (Cannabis sativa L.) is a species considered as having one of the most complicated mechanisms of sex determination. Peroxidase and esterase isoenzymes in leaves of the two sexual phenotypes of hemp were studied. Significant differences in isoperoxidase and isoesterase patterns were found
Increase in cannabis use may indirectly affect the health status of a freshwater species.
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Cannabis is the most used illicit drug worldwide and in some countries a new regulatory policy makes it legal under some restrictions. This situation could lead to a substantial increase in environmental levels of the cannabis active principle (Δ-9-tetrahydrocannabinol [Δ-9-THC]) and its main
Testicular toxicity in cannabis extract treated mice: association with oxidative stress and role of antioxidant enzyme systems.
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Intraperitoneal injection of cannabis extract at low doses (total doses ranging from 40 mg to 60 mg per mouse) induced adverse effect on testes and oxidative stress. At low doses, there was a significant increase in lipid peroxidation and decrease in testicular lipid content, but the effects were
Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid.
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We identified a unique enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid (CBDA) in Cannabis sativa L. (CBDA strain). The enzyme, named CBDA synthase, was purified to apparent homogeneity by a four-step procedure: ammonium sulfate precipitation followed by
Effect of hempseed (Cannabis sativa L.) on performance, egg traits and blood biochemical parameters and antioxidant activity in laying Japanese Quail (Coturnix coturnix japonica).
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1. This study was conducted to determine the effects of hempseed (HS) on performance, egg traits, serum lipid profile and antioxidant activity in Japanese quail (Coturnix coturnix japonica). 2. A total of 120 eight-week-old laying quail were divided into 4 experimental groups with 10 replicates. The
Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress.
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Drought is the main environmental factor impairing hemp growth and yield. In order to decipher the molecular responses of hemp to drought stress, transcriptome changes of drought-stressed hemp (DS1 and DS2), compared to well-watered control hemp (CK1 and CK2), were studied with RNA-Seq technology.
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