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phenylhydrazine/carie dentaire
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Reaction of myoglobin with phenylhydrazine: a molecular doorstop.
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X-ray crystallographic studies of myoglobin do not show an entrance or exit path for potential ligands from the surface to the heme cavity. Efforts to locate such a path have so far centered around dynamic calculations. A structure has now been determined that has a clear opening. Phenylhydrazine
Electron microscopic observations on the development and cytochemistry of the large granule complexes in chicken erythrocyte nuclei.
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Large granule complexes are structures found in a small percentage of chicken erythrocyte nuclei when observed in ultra-thin sections in the electron microscope. They consist of an amorphous region associated with a number of large (approximately 30 min) granules. We have shown, by a novel use of
Erythroid cell growth from normal and W/WV murine bone marrow on macrophage-coated membranes.
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Cellulose acetate membranes (CAM) placed in the peritoneal cavity of mice develop a macrophage layer capable of supporting in vivo hematopoietic colonies from intraperitoneally injected bone marrow cells. Modifications allowing for routine morphologic identification of colonies showed that both
Effect of erythropoietic stimulation of the chamber host on the growth of haemopoietic colonies in plasma clot diffusion chambers (PCDC).
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Murine marrow cells were cultured in Millipore diffusion chambers implanted into the peritoneal cavity of variously conditioned murine hosts. Preirradiation (350 cGy), bleeding (0.5 ml) and phenylhydrazine injection (75 mg/kg i.v.) when performed together on the chamber host, induced better growth
Assessment of erythrocytic and granulocytic colony formation in an in vivo plasma clot diffusion chamber culture system.
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Normal rat bone marrow cells seeded into a plasma clot diffusion chamber culture developed into erythrocytic and granulocytic colonies in vivo. Chambers implanted into the peritoneal cavity of normal hosts showed erythrocytic colony numbers reaching an initial peak on day 2, declining on days 3--5,
The expression of embryonic globin mRNA in a severely anemic mouse model induced by treatment with nitrogen-containing bisphosphonate.
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BACKGROUND
Mammalian erythropoiesis can be divided into two distinct types, primitive and definitive, in which new cells are derived from the yolk sac and hematopoietic stem cells, respectively. Primitive erythropoiesis occurs within a restricted period during embryogenesis. Primitive erythrocytes
Structural insights into the substrate specificity of bacterial copper amine oxidase obtained by using irreversible inhibitors.
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Copper amine oxidases (CAOs) catalyse the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. Although CAOs from various organisms share a highly conserved active-site structure including a protein-derived cofactor, topa quinone (TPQ), their substrate
An exposed tyrosine on the surface of trimethylamine dehydrogenase facilitates electron transfer to electron transferring flavoprotein: kinetics of transfer in wild-type and mutant complexes.
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In wild-type trimethylamine dehydrogenase, tyrosine-442 is located at the center of a concave region on the surface of the enzyme that is proposed to form the docking site for the physiological redox acceptor, electron transferring flavoprotein. The intrinsic rate constant for electron transfer in
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