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phenylhydrazine/hypoxie
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Erythropoietin expressed in granular convoluted tubule cells of mice submandibular glands under hypoxia, anemia, and nephrectomy.
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Immunohistochemically detectable erythropoietin-like substance(Epo) in granular convoluted tubule(GCT) cells of submandibular glands (SMG's) was examined in mice in which hemolytic anemia had been induced by phenylhydrazine (ph), and in mice subjected to hypoxia, nephrectomy, or testosterone (TP)
Direct relationship between radiobiological hypoxia in tumors and monoclonal antibody detection of EF5 cellular adducts.
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While the potential importance of hypoxia in limiting the sensitivity of tumor cells to ionizing radiation has long been appreciated, methods for accurately quantifying the number of radiation-resistant hypoxic cells within tumors have been lacking. We have used the pentafluorinated derivative
Unexpected hypoxia-dependent erythropoietin secretion during experimental conditions not affecting tissue oxygen supply/demand ratio.
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Although a great deal of evidence supports the hypothesis that plasma erythropoietin (EPO) levels of mammals are related to the oxygen supply to the tissues relative to their oxygen needs, several observation millitate against its inherent simplicity. This study presents our results obtained from in
Cardiac remodelling of Atlantic halibut Hippoglossus hippoglossus induced by experimental anaemia with phenylhydrazine.
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Phenylhydrazine injections (0.3 mg kg(-1) , followed by a second injection of 0.1 mg kg(-1) 7 days later) induced a reproducible and stable anaemia in Atlantic halibut Hippoglossus hippoglossus, reducing the haematocrit and haemoglobin by 70.0 and 75.5%, respectively, over 3 weeks. There were no
Hematopoiesis in the embryonic mouse spleen. II. Alterations after phenylhydrazine administration to the mothers.
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The effect of phenylhydrazine (PHZ) on the hematopoietic events in the embryonic spleen of C57Bl/6J mice was examined by light and electron microscopy. Following PHZ in injections to the mothers, the embryonic spleen revealed a marked increase in erythroid precursors, with a shift to mature cells.
[The effect of hypoxia on the glucose-6-phosphate dehydrogenase activity of the erythrocytes in rats].
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Biochemical methods have shown, that adaptation of rats to the conditions of the middle mountains (2100 m above sea level) as well as with subsequent affecting acute hypoxia in the hypobaric chamber (7500 m, 2-5 h and 9000 m, 1-3 h) is accompanied by the increase of the activity of
Stimulation of fetal hemoglobin synthesis in baboons by hemolysis and hypoxia.
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Fetal hemoglobin (Hb F) levels in the peripheral blood of baboons (Papio cynocephalus) increased from an average value of 0.78% to 18.1% during the recovery phase from phenylhydrazine-induced hemolytic anemia. A similar increase was observed in animals exposed to hypobaric hypoxia. Large individual
Iron release, superoxide production and binding of autologous IgG to band 3 dimers in newborn and adult erythrocytes exposed to hypoxia and hypoxia-reoxygenation.
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Iron is released in a desferrioxamine (DFO)-chelatable form when erythrocytes are challenged by an oxidative stress. The release is increased when an accelerated removal of erythrocytes occurs such as in perinatal period, in which iron release is greater in hypoxic than in non-hypoxic newborns. This
Enhanced erythropoietin production during hypobaric hypoxia in mice under treatments to keep the erythrocyte mass from rising: implications for the adaptive role of polycythemia.
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Stress erythropoiesis is usually considered as a compensatory effort to counteract tissue hypoxia. Its homeostatic importance in anemic hypoxia has not been questioned, but researchers, clinicians, and mountain climbers have had second thoughts on polycythemia as to its appropriateness for hypoxic
The effect of the submandibular salivary gland on the erythropoietin response to hypoxia in mice with chronic renal failure.
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The role of the submandibular salivary gland (SG) in the renal and extrarenal erythropoietin (Epo) response to hypoxia was evaluated in adult male mice with chronic renal failure from partial nephrectomy. A partial nephrectomy model for chronic renal failure was used in an attempt to evaluate
Iron metabolism in hepcidin1 knockout mice in response to phenylhydrazine-induced hemolysis.
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Hepcidin, an iron regulatory peptide, plays a central role in the maintenance of systemic iron homeostasis by inducing the internalization and degradation of the iron exporter, ferroportin. Hepcidin expression in the liver is regulated in response to several stimuli including iron status,
Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations.
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The effect of altered oxygen transport potential on behavioural responses to environmental hypoxia was tested experimentally in snapper, Pagrus auratus, treated with a haemolytic agent (phenylhydrazine) or a sham protocol. Standard metabolic rate was not different between anaemic and normocythaemic
Influence of hypoxia and of hypoxemia on the development of cardiac activity in zebrafish larvae.
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Cardiac activity and anaerobic metabolism were analyzed in zebrafish larvae raised under normoxia (PO(2) = 20 kPa) and under chronic hypoxia (PO(2) = 10 kPa) at three different temperatures (25, 28, and 31 degrees C). Heart rate increased with development and with temperature. Under normoxia,
The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation.
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The present study was aimed at determining whether hepcidin, a recently identified peptide involved in iron metabolism, plays a role in conditions associated with both iron overload and iron deficiency. Hepcidin mRNA levels were assessed in two models of anemia, acute hemolysis provoked by
The interactive effects of hypoxemia, hyperoxia, and temperature on the gill morphology of goldfish (Carassius auratus).
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Acclimation of crucian carp and goldfish to temperatures below 15°C causes covering of the gill lamellae by a mass of cells termed the interlamellar cell mass (ILCM). Here we explore the cues underlying gill remodeling (removal or growth of an ILCM) and specifically test the hypotheses that 1)
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