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phosphodiesterase/maïs
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Effect of calmodulin antagonists on auxin-induced elongation.
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Coleoptile segments of oat (Avena sativa var Cayuse) and corn (Zea mays L. var Patriot) were incubated in different concentrations of calmodulin antagonists in the presence and absence of alpha-naphthaleneacetic acid. The calmodulin antgonists (chlorpromazine (CP), trifluoperazine, and fluphenazine)
Correlation between calmodulin activity and gravitropic sensitivity in primary roots of maize.
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Recent evidence indicates a role for calcium and calmodulin in the gravitropic response of primary roots of maize (Zea mays, L.). We examined this possibility by testing the relationship between calmodulin activity and gravitropic sensitivity in roots of the maize cultivars Merit and B73 x Missouri
The lysosome-concept in plants : II. Location of acid hydrolases in maize root tips.
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The sedimentability of eight acid hydrolases in an homogenate from root tips of Zea mays (L.) varied between 13% (α-glucosidase) and 46% (β-N-acetylglucosaminidase) of the total activity. Between 20% and 30% of total activity of the majority of acid hydrolases examined was sedimentable.High specific
Effect of pre- and postnatal essential fatty acid deficiency on brain development and myelination.
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Pregnant mice were fed an essential fatty acid (EFA) deficient diet from day 1 of gestation. Several biochemical parameters of postnatal brain growth and myelination were measured on their progeny and compared with controls fed a normal diet containing 4% corn oil or a commercial breeder diet.
Effect of dietary saturated fatty acids on hormone-sensitive lipolysis in rat adipocytes.
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The objective of this work was to examine the mechanism by which dietary saturated fatty acids, as compared with polyunsaturated fatty acids, lower hormone-sensitive lipolysis in rat adipocytes. Rats were fed a purified diet containing 14% of a fat with a high concentration of either saturated fatty
Role of calmodulin inhibition in the mode of action of ophiobolin a.
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Calmodulin has been isolated from the root of Zea mays. It activates the bovine brain calmodulin-dependent cyclic nucleotide phosphodiesterase and has electrophoretic mobility very similar to that of bovine brain calmodulin. Ophiobolin A, a fungal toxin, interacts with the maize calmodulin. The
Haplo-insufficiency for different genes differentially reduces pathogenicity and virulence in a fungal phytopathogen.
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The main determinant of pathogenicity in Ustilago maydis is the b-mating locus, where establishment of heterozygosity is sufficient to cause galls/tumors on maize plants. However, matings between haploids where one partner contains a mutation, in e.g., the smu1 gene, encoding a Ste20-like PAK
Anti-lipase and lipolytic activities of ursolic acid isolated from the roots of Actinidia arguta.
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The aim of this study was to investigate the anti-obestic effects of ursolic acid isolated from the roots of Actinidia arguta, as well as the mechanism of action of this compound. This was conducted by testing whether ursolic acid inhibited the elevation of the rat plasma triacylglycerol levels
Cyclic diguanylate turnover mediated by the sole GGDEF/EAL response regulator in Pseudomonas putida: its role in the rhizosphere and an analysis of its target processes.
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GGDEF and EAL/HD-GYP protein domains are responsible for the synthesis and hydrolysis of the bacterial secondary messenger cyclic diguanylate (c-di-GMP) through their diguanylate cyclase and phosphodiesterase activities, respectively. Forty-three genes in Pseudomonas putida KT2440 are putatively
A new method for the quantification of aortic calcification by three-dimensional micro-computed tomography.
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To gain a better understanding of the mechanisms that underpin aortic calcification, rodent models have been previously utilised. Regions of calcium and phosphate deposition are commonly visualised using labor-intensive two-dimensional histomorphometric techniques. In this study, we developed a
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