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Page 1 de 4538 résultats
PSC-RED and MNC-RED: Albumin-free and low-transferrin robust erythroid differentiation protocols to produce human enucleated red blood cells.
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Many methods have been developed to produce cultured red blood cells (cRBCs) in vitro but translational applications have been hampered by high costs of production and by low rates of enucleation. We have developed R6 and IMIT, two chemically defined culture media and combined them into robust
Reduced red cell filtrability due to red cell plasma protein interactions.
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The adsorption of protein on red cell surface was studied by the measurement of protein in plasma and incubation of blood with 131I-albumin. The protein concentration of the moderately hypertonic plasma clearly decreased by the addition of red cells. The decay counts of 131I-albumin increased in the
Haemotherapy with red-cell concentrates and a new red-cell storage medium.
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The effects on plasma proteins and haemostasis of haemotherapy with buffy-coat-poor red-cell concentrate and red cells suspended and stored in a new medium containing sodium chloride, adenine, glucose, and mannitol (SAGM) were studied in elective surgery. In patients with normal preoperative serum
Red cell-mediated microinjection.
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I have reviewed the current status of microinjection based on fusion of red blood cells and tissue culture cells. Macromolecules are introduced into red blood cells during hypotonic hemolysis, and the resealed red cells are then fused to tissue culture cells with Sendai virus. The procedure has been
SIMULTANEOUS RED CELL MASS AND PLASMA VOLUME DETERMINATIONS USING 51-CR TAGGED RED CELLS AND 125-I LABELED ALBUMIN.
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Red cell, plasma and albumin transfusion decision triggers.
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Hypothetical clinical cases were used to investigate transfusion-related decision-making. Three red cell, three fresh frozen plasma (FFP) and three albumin transfusion decision cases were administered by questionnaire to 228 medical staff. The transfusion decision triggers were identified and
Comparison of red cell and whole blood volume as performed using both chromium-51-tagged red cells and iodine-125-tagged albumin and using I-131-tagged albumin and extrapolated red cell volume.
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BACKGROUND
Our facility's current blood volume measurement protocol has involved separate measurement of plasma and red cell volumes. The purpose of this study is to determine whether measurement with a recently FDA-approved, one-compartment semiautomated system provides similar
Red cell and albumin circulations in the ileum.
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Binding of phenol red by human serum albumin.
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Simplified method for simultaneous determinations of plasma volume and red-cell mass with 125I-labeled albumin and 51Cr-tagged red cells.
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Fluorescence Red-edge-excitation Effects of Human Serum Albumin.
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We have studied the fluorescence red-edge-excitation effects of human serum albumin. Our results indicate that the red-edge-excitation effects of human serum albumin depend on the pH, temperature and concentration of the denaturant. The different red-edge-excitation effects are produced by a
Letters to the Editor. Simultaneous red cell mass and plasma volume determinations using Cr51 tagged red cells and I-125 labeled albumin.
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Red blood cells under mechanical stress.
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The effect of mechanical stress on erythrocytes suspended in various media was studied. The ability of the cells to increase their glucose consumption was found to be the major criterion allowing to divide the media into two groups. In plasma, serum or in Ringer's solution supplemented with albumin
Genetic studies of water buffalo blood markers. I. Red cell acid phosphatase, albumin, catalase, red cell alpha-esterase-3, group-specific component, and protease inhibitor.
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We have developed the methodologies for typing and family studies to establish the modes of inheritance of water buffalo red cell acid phosphatase (Acp), protease inhibitor (Pi), and group-specific component (Gc) on isoelectric focusing and albumin (Alb), red cell alpha-esterase-3 (Est-3), and
Red blood cells protect albumin from cigarette smoke-induced oxidation.
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Different studies reported the presence of oxidized (carbonylated) albumin in the extravascular pool, but not in the intravascular one of cigarette smokers. In this study we attempted to explain this apparent discrepancy exposing human serum albumin (HSA) to aqueous cigarette smoke extract (CSE).
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