Page 1 de 1440 résultats
Five compounds related to Congo Red were found to inhibit generation of protease-resistant prion protein in a cell-free system. In this assay Trypan Blue, Evans Blue, Sirius Red F3B, Primuline and Thioflavin-S were all more inhibitory than Congo Red itself. In scrapie-infected mouse neuroblastoma
Studies of malaria proteases have focused on two general groups, corresponding to activities specific to malaria parasites: (1) proteases involved in hemoglobin degradation which are active in the food vacuole and which exhibit optimal activity at low pH; and (2) proteases specific to schizonts
The discrimination between erythrocyte and Plasmodium proteases is now made easier by using synthetic fluorogenic substrates, high-pressure liquid chromatography, reliable methods of cell preparation, as well as radiolabeled extracts from in vitro cultures of P. falciparum. The reinvasion process of
The study of the agglutinability of Xg(a + ) cells by several examples of anti-Xga and absorption-elution tests showed that the red blood cell antigen Xga is destroyed by proteases commonly used in blood group serology but not by neuraminidase.
Release of vesicles from human red cell membranes was induced either by ATP-depletion or by incubation of the cells in presence of sonicated dimyristoylphosphatidylcholine (DMPC) vesicles. Vesicles released from ATP-depleted red cells but not the DMPC-induced vesicles contained degradation products
We have developed a novel red fluorescent dye, 2Me SiR600 (λ(em)=613 nm), in which the O atom of Rhodamine Green at the 10 position of the xanthene moiety is replaced with a Si atom, as a scaffold for probes to detect protease activity with extremely high S/N ratio. As proof of concept, we designed
Aprotinin and alpha 1-proteinase inhibitor have been encapsulated in human red blood cells (RBC) by a dialysis technique that involves transient hypotonic haemolysis followed by isotonic resealing. Both protease inhibitors can be encapsulated to a considerable extent. These molecules are released
Acetylcholinesterase activity and molecular groups characteristic of erythrocyte ghost membrane proteins show significant circadian oscillations over 24 h. These fluctuations were previously interpreted as very slow oscillations with a period ranging between 1.3 and 1.6 h. Autocorrelation function
Pyropia yezoensis, one of the most economically important marine algae, suffers from the biotic stress of the oomycete necrotrophic pathogen Pythium porphyrae. However, little is known about the molecular defensive mechanisms employed by Pyr. yezoensis during the infection
ZZAP is a mixture of a sulfhydryl reagent (dithiothreitol) and a proteolytic enzyme (papain or ficin). This reagent dissociates IgG and complement from red blood cells, allowing for phenotyping, enhanced adsorption, or denaturing of multiple blood group system antigens to aid in completing complex
The thalassemias are extremely heterogeneous in terms of their clinical severity, and their underlying pathophysiology relates directly to the extent of accumulation of excess unmatched globin chains: alpha in beta thalassemia and beta in the alpha thalassemias. However, the accumulation of each
We have developed the methodologies for typing and family studies to establish the modes of inheritance of water buffalo red cell acid phosphatase (Acp), protease inhibitor (Pi), and group-specific component (Gc) on isoelectric focusing and albumin (Alb), red cell alpha-esterase-3 (Est-3), and
Invasion of red blood cells by the malaria merozoite is an essential step in the life cycle of this obligate intracellular pathogen. The molecular details of invasion are only recently becoming understood, largely through studies in related apicomplexan parasites such as Toxoplasma. Protease
Congo red inhibits the accumulation of protease-resistant PrP in scrapie-infected mouse neuroblastoma cells. Here we show that Congo red also inhibits the replication of scrapie infectivity in these cells. This observation is consistent with the idea that protease-resistant PrP is a vital component