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solasodine/solanum
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Solasodine glycosides of Solanum unguiculatum (A.) Rich.
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Besides solasonine, three new glycosides, namely, 3-O-alpha-L-rhamnopyranosyl-(1-->3)-solasodine, 3-O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-rhamnopyranosyl-(1-->4)-beta -D- galactopyranosyl solasodine, and 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-galactopyranosyl solasodine, were isolated from
Solasodine production from self-immobilised Solanum aviculare cells.
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Procedures were developed for 'self-immobilisation' of Solanum aviculare cells to eliminate the need for artificial immobilisation supports. Depending on the cytokinin used in liquid medium, compact aggregates 0.4-2.0 cm in diameter were formed without dispersed cells also being present.
Anticonvulsant activity of solasodine isolated from Solanum sisymbriifolium fruits in rodents.
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BACKGROUND
Solanum sisymbriifolium Lam. (Solanaceae), commonly known as sticky nightshade, is traditionally used for central nervous system (CNS) disorders. Although solasodine has been isolated from this plant, little is known about its anticonvulsant and CNS depressant actions.
OBJECTIVE
We
[Induction and in vitro culture of hairy roots of Solanum nigrum L. var. pauciflorum Liou and its solasodine production].
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By using genetic transformation of Agrobacterium rhizogenes and liquid culture, induction and culture conditions for Solanum nigrum L. var. pauciflorum Liou hairy roots and its solasodine production and consumption changes of N resource and calcium in the medium during liquid culture were
Solasodine-3-O-β-d-glucopyranoside kills Candida albicans by disrupting the intracellular vacuole.
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The increasing incidence of fungal infections and emergence of drug resistance underlie the constant search for new antifungal agents and exploration of their modes of action. The present study aimed to investigate the antifungal mechanisms of solasodine-3-O-β-d-glucopyranoside (SG) isolated from
The regulation of solasodine production by Agrobacterium rhizogenes-transformed roots of Solanum aviculare.
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Transgenic roots of Solanum spp. containing extra copies of an hmgr gene derived from Artemisia annua have been obtained via transformation with Agrobacterium rhizogenes. Hairy root clones of Solanum aviculare which were transgenic for hmgr typically grew faster than those which did not contain
Effect of grafting on solasodine content in Solanum viarum.
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Solasodine--a steroid present in the mucilaginous exotesta of the seed in Solanum viarum Dunal (syn. S. khasianum var. chatterjeeanum) is a major source of steroidal raw material. Evidence for the site of solasodine synthesis was sought through reciprocal grafts involving steroid-bearing S. viarum
Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species.
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The development of sequential injection analysis with lab-at-valve (LAV) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of
Evaluation of chemical analysis for the determination of solasodine in Solanum laciniatum.
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A detailed study of a method for solasodine analysis has been carried out and the suitability of chemical analysis for solasodine determination in plant material evaluated. A number of problems with the analytical isolation of solasodine and its subsequent colorimetric determination have been
The effect of solasodine on the body temperature.
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The action of solasodine, the steroid-structure alcaloid of Solanum laciniatum Ait., was studied on normal and pyretic body temperature of rats and mice. In rats a single dosage of 3 mg/kg depressed by an average of --1.5+/-0.3 degrees C the normal temperature for 24 h. With larger dosages or longer
Solasodine levels in Solanum sisymbriifolium Lam.
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Solasodine content in berries, leaves and stem of S. sisymbriifolium, using three different procedures, was 0.93, 0.73 and 0.23% respectively. Further in chromatogram not only solasodine but three more alkaloids (Rf 0.22, 0.48 and 0.88) were found which could not be identified due to lack of
Genetic transfection, hairy root induction and solasodine accumulation in elicited hairy root clone of Solanum erianthum D. Don
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An efficient genetic transfection technique has been established using A4 strain of Agrobacterium rhizogenes for the first time in a medicinally valuable plant Solanum erianthum D. Don. The explants were randomly pricked with sterile needle, inoculated with bacterial suspension. The infected leaves
Estimation of glycoalkaloids as solasodine in Solanum laciniatum.
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Solasodine is isolated from Solanum laciniatum leaves by a rapid, one-step procedure which involves extraction and hydrolysis of the glycoalkaloids. The resulting steroidal aglycone is directly estimated by the formation of a colored complex with bromocresol green. The method is applicable to
Solasodine production in rapidly proliferating tissue cultures of Solanum laciniatum ait.
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Callus and suspension cultures, established from seedling and leaf explants of Solanum laciniatum Ait were analysed for solasodine using a spectrophotometric assay. Solasodine concentration in both types of culture ranged from 0.5 - 1.0 mg/g dry wt., with a small number of callus explants containing
Production of solasodine by Solanum laciniatum using plant tissue culture technique.
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Leaf and hypocotyl explants of 15 days old aseptically grown seedlings of Solanum laciniatum were cultured on MS medium supplemented with NAA (2 mg/l) and kinetin (0.5 mg/l) for callus initiation. For maintenance and proliferation of callus MS medium supplemented with 2,4-D (1 mg/l) and kinetin (0.5
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