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13 résultats
Functional analysis of TcCTLP-5C2, a chymotrypsin-like serine protease needed for molting in Tribolium castaneum.
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In a previous study, we have characterized a gene family encoding chymotrypsin-like proteases from the red flour beetle, Tribolium castaneum (TcCTLPs). We identified 14 TcCTLP genes that were predominantly expressed in the midgut, where they presumably function in digestion. Two genes (TcCTLP-6C and
Microarray analysis reveals strategies of Tribolium castaneum larvae to compensate for cysteine and serine protease inhibitors.
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The transcriptome response of Tribolium castaneum larvae to dietary protease inhibitors was evaluated by whole-genome microarray analysis. RNA was isolated from guts of larvae fed control diet (no inhibitor), or diets containing 0.1% E-64 (cysteine protease inhibitor), 5.0% soybean trypsin inhibitor
Separation of a Tribolium-protease inhibitor from soybeans on a calcium phosphate column.
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Insecticidal Activity of Artemisia vulgaris Essential Oil and Transcriptome Analysis of Tribolium castaneum in Response to Oil Exposure
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Red flour beetle (Tribolium castaneum) is one of the most destructive pests of stored cereals worldwide. The essential oil (EO) of Artemisia vulgaris (mugwort) is known to be a strong toxicant that inhibits the growth, development, and reproduction of T. castaneum. However, the
Influence of Different Food Commodities on Life History, Feeding Efficiency, and Digestive Enzymatic Activity of Tribolium castaneum (Coleoptera: Tenebrionidae).
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The life history, feeding indices, and digestive enzymatic activity of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) were studied on flours of 10 commodities (artificial diet, barley, cowpea, maize, millet, rice, sorghum, soybean, triticale, and wheat) at 30 ± 1°C, relative humidity 75 ±
Improvement of the Insecticidal Capacity of Two Purpureocillium Lilacinum Strains against Tribolium Confusum.
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Entomopathogenic fungi can regulate insect populations. They have extracellular enzymes that degrade cuticle components, mainly hydrocarbons, used as an energy source. The increase in insecticidal activity of fungi in a medium supplemented with cuticular hydrocarbons was assayed and the hydrolytic
[Some properties of proteolytic enzymes from Tribolium confusum].
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The digestive tract of Tribolium confusum Duv. larvae was studied for proteolytic enzymes properties. The pH optima are determined for the enzymes effect on various substrates. Proteases were partially purified by gel chromatography on Sephadex G = 100 and investigated for thyol compound influence
Transcriptome profiling analysis reveals the role of latrophilin in controlling development, reproduction and insecticide susceptibility in Tribolium castaneum.
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Latrophilin of Tribolium castaneum (Tclph) has been reported to play crucial roles in growth, development and reproduction. However, the regulatory mechanism of Tclph associated with these physiology processes is unknown. Thus, the global transcriptome profiles between RNAi treated (ds-Tclph) and
Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum.
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BACKGROUND
Phosphine is a valuable fumigant to control pest populations in stored grains and grain products. However, recent studies indicate a substantial increase in phosphine resistance in stored product pests worldwide.
RESULTS
To understand the molecular bases of phosphine resistance in
Comparative RNA-sequencing analysis of ER-based HSP90 functions and signal pathways in Tribolium castaneum.
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Tribolium castaneum, the red flour beetle, is a major agriculture pest that damages stored grains and cereal products. Heat-shock protein 90 of T. castaneum (Tchsp90) has been reported to play pivotal roles in heat stress response, development, reproduction, and life span. However, the signaling
Characterisation and tissue distribution of the PISCF allatostatin receptor in the red flour beetle, Tribolium castaneum.
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The insect PISCF/allatostatins (ASTs) are pleiotropic peptides that are involved in the regulation of juvenile hormone biosynthesis, are myoinhibitory on the gut and the heart, and suppress feeding in various insects, but their roles in beetles are poorly understood. To provide further insight into
Proteomics of Tribolium castaneum seminal fluid proteins: identification of an angiotensin-converting enzyme as a key player in regulation of reproduction.
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Seminal fluid proteins (SFPs) play important roles in regulation of reproduction and behavior. Proteomics approaches were used to identify 13 SFPs, including 3 new proteins in the red flour beetle, Tribolium castaneum. The 13 SFP genes identified code for Serpin, cysteine-rich protein, odorant
RNA interference and dietary inhibitors induce a similar compensation response in Tribolium castaneum larvae.
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Tribolium castaneum is a major agriculture pest damaging stored grains and cereal products. The T. castaneum genome contains 26 cysteine peptidase genes, mostly cathepsins L and B, and seven have a major role in digestion. We targeted the expression of the most highly expressed cathepsin L gene on
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