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Ulex europaeus I and glycine max bind to the human olfactory bulb.
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The distribution of binding sites for the fucose-selective lectin Ulex europaeus I and the terminal N-acetylgalactosamine-selective lectin glycine max in the human olfactory bulb were studied. These lectins bound to primary olfactory axons in the olfactory nerve layer and the glomerular layer. They
Demonstration of sugar residues in the ultimobranchial tubule and thyroid C-cells of the rat using peroxidase labelled lectins.
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The distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid C-cells has been studied, using eight peroxidase-labelled lectins (Dolichos biblorus, Glycine max, Bauhinia purpurea, Helix pomatia,
Lectin binding patterns in developing rat colon.
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To evaluate qualitative changes in brush-border and goblet cell glycoconjugates during colonic development, rhodamine-conjugated lectins were applied to tissue from fetal (18-22 day), suckling (1-20 day), and weanling (22-26 day) rats. Tissue from the proximal and distal colon of each animal was
Detection of surface carbohydrates on Pneumocystis carinii by fluorescein-conjugated lectins.
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Lectins react with a wide range of different carbohydrates (Table 1). Even so-called monospecific anti-H(O) lectins from Lotus tetragonolobus, Ulex europaeus, and Anguilla anguilla react not only with the anti-H determinant but also with several fucosylated carbohydrates. Consequently, the type of
Dynamic changes of cell-surface glycoconjugates in human palmar epidermis following friction-blisters.
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Damage and repair of cell-surface glycoconjugates were examined in human palmar skin following friction-blister injury, using biotinylated lectins and the avidin-biotin complex method. In normal skin, concanavalin A, Ricinus communis, and Triticum vulgaris bound to the surface of cells from the
Lectin binding sites on human endocervix: a comparison with secretory and proliferative endometrium.
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Endocervix and corresponding endometrium of women of reproductive age were studied histochemically with 13 fluorescein isothiocyanate-labeled lectins to delineate the differences between the epithelial cells in two anatomical sites. Lectin from Maclura pomifera (MPA), Ulex europaeus (UEA-I), Glycine
Lectin binding to small intestinal goblet cells of newborn, suckling, and weaned pigs.
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Lectin binding of small intestinal goblet cells was examined in newborn, suckling, and weaned pigs. Sections of duodenum, proximal portion of the jejunum, distal portion of the jejunum, and ileum were embedded in a hydrophilic acrylic resin and treated with each of the following lectins: Canavalia
High osmotic pressure for Pneumocystis carinii London Resin White embedding enables fine immunocytochemistry studies: I. Golgi complex and cell-wall synthesis.
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A method for embedding Pneumocystis carinii in hydrophilic resin (London Resin White) has been developed for immunocytochemistry studies. Using high osmotic pressure (about 850 mosmol) from fixation to embedding, this method improved the preservation of the fine structure as well as the antigenicity
Histochemical study on the bile duct system of normal rats.
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The bile duct system of normal rats was examined histochemically. Although the apical surface of biliary and glandular epithelial cells was positively stained with Concanavalia ensiformis (Con A), Dolichos biflorus (DBA), Glycine max (SBA), Ulex europeus-I (UEA-I) and Triticumvulgaris (WGA), the
Exposed and hidden lectin-binding epitopes at the surface of Borrelia burgdorferi.
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The presence of surface- and subsurface-located lectin-binding epitopes of Borrelia burgdorferi was examined by electron microscopy using a variety of gold-labeled lectins. Concanavalin A reacted predominantly with extracellular material adjacent to the spirochetes. Wheat germ agglutinin bound
Lectin binding sites in parotid acinar secretory granules of normal and isoproterenol treated rat.
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Lectin staining patterns in secretory granules of rat parotid gland acinar cell of untreated and isoproterenol-injected animals were examined by electron microscopy. We used four lectin-gold complexes: Ulex europaeus agglutinin I (UEA-I), Helix pomatia agglutinin (HPA), wheat germ agglutinin (WGA),
Human sperm surface mapping with lectins.
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Ten fluorescein isothiocyanate (FITC)-linked lectins [Bauhimia purpurea, Concanavalin A, Dolichos biflorus (DBA), Griffonia simplicifolia I, Griffonia simplicifolia II, Maclura pomifera, Arachis hypogea (PNA), Glycine max, Ulex europaeus (UEA) and Triticum vulgaris agglutinin] have been used to
Lectin binding patterns in amphibian epidermis.
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A battery of 6 different horseradish peroxidase conjugated lectins has been employed for structural localization of glycoconjugates in amphibian epidermis. Lens culinaris (LCA) lectin stained the basal membrane and gave no significant reaction on the epidermal layers. Canavlia ensiformis (Con A) and
Characterization of glycoconjugates in the endometrium of the miniature pig during different reproductive periods.
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Lectin-binding glycoconjugates in the endometrium of miniature pigs were determined for sexually immature, dioestrus, oestrus and early pregnancy periods, using five horseradish peroxidase-labelled lectins. Apparent changes associated with sexual maturity were increases in binding intensity of wheat
Differential localization of lectin binding sites and neuropeptides in human dorsal root ganglia.
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The subpopulations were compared of neurons in human dorsal root ganglia (DRG), as substance P, identified by somatostatin, Glycine max lectin (SBA) specific to terminal N-acetylgalactosamine, and Ulex europaeus I agglutinin (UEA-I) specific to L-fucose. The lectins and neuropeptides all bound to
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