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The induction of tumors in the hybrid Nicotiana glauca x N. langsdorffii plants by 6-azauracil and its reversal by uracil and actinomycin D.
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Effect of base, pentose, and phosphodiester backbone structures on binding and repair of pyrimidine dimers by Escherichia coli DNA photolyase.
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Photolyases reverse the effects of UV light on cells by converting cyclobutane dipyrimidine photoproducts (pyrimidine dimers, Pyr mean value of Pyr) into pyrimidine monomers in a light-dependent reaction. Previous work has suggested that, based on substrate preference, there are two classes of
Amino acid residues in ribonuclease MC1 from bitter gourd seeds which are essential for uridine specificity.
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The ribonuclease MC1 (RNase MC1), isolated from seeds of bitter gourd (Momordica charantia), consists of 190 amino acids and is characterized by specific cleavage at the 5'-side of uridine. Site-directed mutagenesis was used to evaluate the contribution of four amino acids, Asn71, Val72, Leu73, and
Flower Formation in Excised Tobacco Stem Segments; II. Reversible Removal of IAA Inhibition by RNA Base Analogues.
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The RNA base analogues, 2-thiouracil, 6-azauracil and 8-azaguanine incorporated singly into the medium, increased the number of floral buds in excised stem segments of Nicotiana tabacum variety Wisconsin No. 38 cultured in vitro. Combined treatments with 2 and 3 base analogues were even more
Mode of inheritance and evidence for cistron heterogeneity of chloroplast 16S ribosomal RNA genes in Nicotiana.
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Oligonucleotide maps (fingerprints) of T1 RNase digests of 125I-labeled 16 S chloroplast rRNA of Nicotiana tabacum and N. gossei revealed the presence of T1 oligonucleotide fragment 100 in the 16 S rRNA of N. gossei while N. tabacum 16 S rRNA had a unique T1 oligonucleotide (fragment 101) as well as
Analogues of pyrimidine base precursors as antiphytoviral agents.
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Dihydroorotic acid hydrazide (DHOH) did not inhibit the replication of potatovirus X (PVX) in leaf disks of Nicotiana tabacum 'Samsun'. In contrast, 5-fluoroorotic acid (5-FOA) completely inhibited the replication of PVX, as demonstrated by a serological virus assay as well as a local lesion
Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5'-GMP: structural basis for alterations in substrate specificity.
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Ribonuclease MC1 (RNase MC1), isolated from bitter gourd seeds, is a uridine specific RNase belonging to the RNase T2 family. Mutations of Asn71 in RNase MC1 to the amino acids Thr (N71T) and Ser (N71S) in guanosine preferential RNases altered the substrate specificity from uridine specific to
Growth inhibition of tobacco tissue cultures with 6-azauracil, 6-azauridine and maleic hydrazide.
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Tissue cultures of Nicotiana tabacum, Nicotiana suaveolens and Nicotiana suaveolens x Nicotiana langsdorffii were cultured in the presence of the growth inhibitors maleic hydrazide and 6-azauracil as well as 6-azauridine. Inhibition of growth was complete at 10(-4) molar concentrations in all 3
A point mutation in the tobacco mosaic virus capsid protein gene induces hypersensitivity in Nicotiana sylvestris.
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In Nicotiana sylvestris, the N' gene confers hypersensitive resistance to some strains of tobacco mosaic virus (TMV) but not to the common strain. TMV sequences responsible for inducing local lesion formation in this host were identified by using cDNA clones to construct genomic recombinants between
Mode of action of the antiphytoviral compound 2,4-dioxohexahydro-1,3,5-triazine (5-azadihydrouracil).
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The time course of inhibition of potato virus X ( PVX ) synthesis by the newly developed antiphytoviral compound 2,4- dioxohexahydro -1,3,5-triazine (DHT) was determined in mechanically inoculated leaves of Nicotiana tabacum L. cv. ' Samsun '. At the permissive temperature (22 +/- 3 degrees) DHT
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