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Uridine-induced hyperthermia in the rabbit.
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Uridine injection in 0.6% saline elevated rabbit temperatures (mean = 0.9 degree C) in the USP XX pyrogen test. Hyperthermia was delayed in onset and peaking 3-4 h post injection, but the injection was negative in the limulus amoebocyte lysate (LAL) assay. Uridine from five lots of different sources
Diffusion kinetics of tritiated uridine into HeLa cells previously exposed to hyperthermia.
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Hyperthermic treatment of HeLa cells at 42 degrees C for 60 min depressed the specific activity of these cells when incubated with 3H-uridine both during and post heating compared to cells maintained at 37 degrees C. These changes were unlikely to arise from increased leakage from the cells and may
In vitro antiviral activity of some uridine derivatives of 2-deoxy sugars against classical swine fever virus.
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Classical swine fever virus glycoproteins: E2, E(rns) (E0) and E1 are detected on the external part of viral particles and play a major role in the initial stages of viral infection. They form heterodimeric and homodimeric complexes needed to effectively infect host cells. Some glycosylation
Novel poly-uridine insertion in the 3'UTR and E2 amino acid substitutions in a low virulent classical swine fever virus.
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In this study, we compared the virulence in weaner pigs of the Pinar del Rio isolate and the virulent Margarita strain. The latter caused the Cuban classical swine fever (CSF) outbreak of 1993. Our results showed that the Pinar del Rio virus isolated during an endemic phase is clearly of low
A poly-uridine insertion in the 3'-untranslated region of classical swine fever virus activates immunity and reduces viral virulence in piglets.
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Low virulent classical swine fever virus (CSFV) strains make CSF eradication particularly difficult. Little data is available on the molecular determinants of CSFV virulence. The aim of present study is to assess a possible role for CSFV virulence of a unique uninterrupted 36-uridine (poly-U)
[Changes caused by uridine amphetamine induced hyperthermia in mice and rats].
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Incorporation of [3H] uridine by mouse embryos with abnormalities induced by parental hyperthermia.
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Changes in hepatic glycogen, protein, and ribonucleic acid synthesis, and some effects of cortisol, during Q fever.
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Liver glycogen is depleted in guinea pigs infected with Coxiella burneti. Syntheses of the glycogen precursors uridine triphosphate and uridine diphosphate glucose are unaffected during Q fever, but glycogen synthetase activity is inhibited. Exogenous cortisol relieves this inhibition in infected
Secreted expression of the classical swine fever virus glycoprotein E(rns) in yeast and application to a sandwich blocking ELISA.
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E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) with RNase activity. The purpose of this study was to produce an active E(rns) for further applications using the yeast secreted expression system. The E(rns) gene was cloned into the expression vector pGAPZalphaC which was
Clinical and pharmacokinetic studies of prolonged administration of high-dose uridine intended for rescue from 5-FU toxicity.
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A clinical and pharmacokinetic investigation of prolonged administration of high-dose uridine was performed in seven patients with advanced-stage cancer. Uridine administration was examined as a continuous infusion at 1 and 2.5 g/m2/hr (two patients) and as a series of intermittent infusions during
Antiviral activity of uridine 5'-diphosphate glucose analogues against some enveloped viruses in cell culture.
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Twenty five analogues of uridine 5'-diphosphate glucose were screened against herpes simplex type 2, vaccinia virus, Sindbis virus and African swine fever virus. After screening, the compound 5'-[[[[(2",3",4",6"-tetra-O-benzoyl-alpha-D- glucopyranosyl)oxi]carbonyl]amino]sulfonyl]uridine (2), the
[Pharmacology of uridine and cytidine].
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It was established that with intraperitoneal introduction of uridine and cytidine their DL50 for mice equals 5100 and 2700 mg/kg, respectively. In doses of 1/27 and 1/50 of DL50 cytidine reduces by 50 per cent the edema of the rat's paw in a dextran-and formaldehyde-induced inflammation, brings down
[Effect by proliferation kinetics of malignant tumors of children of hyperthermia].
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Embryonal tumors (21 Wilms' tumors, 11 neuroblastomas, 9 rhabdomyosarcomas) and 16 sarcomas of the skeleton of childhood were studied with an autoradiographic in vitro method according to the responsibility to hyperthermia 42.5 degrees C/120 min, to Cyclophosphamide, to Doxorubicin, and to
[In vitro studies of DNA synthesis inhibition by hyperthermia (42.5 degrees C, 120 min) and cytostatic drugs in embryonal tumors and sarcomas in childhood].
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The effect of hyperthermia (HT), (42.5 degrees C, 120 min), cytostatic agents and the simultaneous application of HT and cytostatics on embryonal tumors and sarcomas in children was studied using an autoradiographic in vitro method. The 3H-thymidine and 3H-uridine incorporation inhibition was
Exchanging the yellow fever virus envelope proteins with Modoc virus prM and E proteins results in a chimeric virus that is neuroinvasive in SCID mice.
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A chimeric flavivirus infectious cDNA was constructed by exchanging the premembrane (prM) and envelope (E) genes of the yellow fever virus vaccine strain 17D (YF17D) with the corresponding genes of Modoc virus (MOD). This latter virus belongs to the cluster of the "not-known vector" flaviviruses and
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