[A preliminary study on tissue culture of stem apex of Chrysanthemum morifolium].

To solve the breed degeneration of Chrysanthemum the stem apex of Chrysanthemum morifolium used as explants were inoculated on MS medium with different pH and supplemented with different ratio of plant hormone. The results showed that when pH was between 5.8 and 6.4, the rate of callus were all over 80%. While explants inoculated on MS medium supplemented with NAA 0.2 mg/L and KT 0.1-0.2 mg/L, the rate of callus was noticeably higher than that of NAA 0.2 mg/L and 6-BA 1-3 mg/L. The preferable medium which roots could be induced was 1/2 MS + NAA 0.2 mg/L.