Callus Proliferation and Plant Recovery with Oxalis erosa Knuth Shoot Tip Cultures.

A successful micropropagation method for Oxalis erosa Knuth is reported. The most suitable media contained Murashige and Skoog mineral salts and carbohydrates, including (M): 0.6 × 10(-6) thiamine-HCl, 4.8 × 10(-6) pyridoxine-HCl and 8.2 × 10(-5) nicotinic acid, and supplements of (M): 4.9 × 10(-7) IBA + 4.4 × 10(-6) BAP + 2.9 × 10(-7) GA(3) for callus induction, 2.2 × 10(-6) BAP + 2.9 × 10(-7) GA(3) for bud differentiation and shoot proliferation, 2.6 × 10(-6) GA(3) for internode elongation and leaf blade expansion, and the BM at half-strength + 2.5 × 10(-8) IBA, agar omitted, for the rooting of shoots.