[[Effect of lanthanum chloride on cytokines expression of RAW264.7 induced by alumina ceramic particles].

OBJECTIVE

To investigate the relationship between alumina ceramic particles and aseptic loosening of the joint prosthesis and the effect of lanthanum chloride on the secretion of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) of macrophage RAW264.7 induced by alumina ceramic particles.

METHODS

RAW264.7 cells were cultured in vitro and divided randomly into 4 groups according to different culture solutions: blank control group (group A), 1 mg/mL alumina ceramic particles (group B), 1 mg/mL alumina ceramic particles and 10 μmol/L lanthanum chloride (group C), and 10 μmol/L lanthanum chloride (group D). The cell growth was detected by MTT, and ELISA, RT-PCR, and Western blot were used to test the expressions of IL-1β, TNF-α, and nuclear factor κB (NF-κB).

RESULTS

There was no significant difference in cell growth among all groups by MTT (F = 2.180, P = 0.142). RT-PCR results showed that the expressions of IL-1β, TNF-α, and NF-κB mRNA in group B were significantly higher than those in the other 3 groups (P < 0.05); the expressions in group D were significantly lower than those in group A (P < 0.05). ELISA results showed that the contents of IL-1β and TNF-α in group B were significantly higher than those in the other 3 groups (P < 0.05); the contents in group D were significantly lower than those in group A (P < 0.05). Western blot analysis revealed that the expression of NF-κB protein in group B was significantly higher than that in the other 3 groups (P < 0.05).

CONCLUSIONS

Alumina ceramic particles can stimulate the secretion of IL-1β and TNF-α of macrophage, and lanthanum chloride can inhibit the secretion of IL-1β and TNF-α of macrophage.