Characterisation of lanthanum toxicity for root growth of Arabidopsis thaliana from the aspect of natural genetic variation

The mechanism of lanthanum (La³⁺) toxicity on root growth of Arabidopsis was studied by physiological and genetic approaches using Landsberg erecta (Ler) × Columbia (Col) recombinant inbred lines (RILs) and other natural accessions. Quantitative trait locus (QTL) analyses revealed regulation of La³⁺ tolerance of the Ler × Col RILs by multiple genetic factors consisted of three significant QTLs and seven epistatic interacting loci pairs. The La content in the root tip was not correlated with La³⁺ tolerance in the RILs, indicating that the observed La³⁺ rhizotoxicity was not related to direct toxicity of La³⁺ in the symplast. The La³⁺ tolerance of root growth in the RILs was not correlated with Al³⁺ and Cu²⁺ tolerances, but was correlated with tolerances for other rare earth elements, including Gd³⁺, a known Ca²⁺ channel antagonist, and verapamil, a Ca²⁺ channel blocker. The genetic architecture of verapamil tolerance in root growth, which was identified by QTL analysis, was closely related to that of La³⁺ tolerance. La³⁺ tolerance and verapamil tolerance or Gd³⁺ tolerance in natural accessions of Arabidopsis also showed a positive correlation. These results indicate that the major La³⁺ toxicity on the root growth of Arabidopsis may involve its action as a Ca²⁺ channel antagonist.