In vitro antitubercular, anticancer activities and IL-10 expression in HCT-116 cells of Tinospora sinensis (Lour.) Merr. leaves extract.
कीवर्ड
सार
Considering the importance of Tinospora sinensis in folklore as immunomodulant, the leaves subjected to cold maceration using ethanol, ethyl acetate and n-hexane and then screened for their antitubercular, anticancer and IL-10 expression (HCT-116) properties using luciferase reporter phage, MTT and flow cytometry assay methods, respectively. The results revealed that ethyl acetate and n-hexane extracts demonstrated the promising (p < 0.05) antitubercular activity with inhibition (%) of 92% and 86%, at 100 µg/ml, respectively and the respective IC50 values of 63.99 and 113.7 µg/ml on HeLa and HCT-166 cells. The n-hexane extract demonstrated the significant (p < 0.05) IL-10 expression (44.12 MFU) in HCT-116 cells against the control cells (8.48 MFU). The GC-MS analyses of n-hexane extract showed presence of tembatarine, berberine, cordifoliside E, and magnoflorine as major constituents. Among all extracts, n-hexane leaf extract was found to be potent against inhibition of colon cancer cells (HCT-116) and Mycobacteria, with significant immunostimulant activity.