[Enzymatic extraction and antibacterial activity of aucubin from Eucommia ulmoides leaves].
Ključne riječi
Sažetak
OBJECTIVE
To investigate the technology of Aucubin in Eucommia ulmoides leaves extracted by enzymatic method and its antibacterial activity.
METHODS
Aucubin in Eucommia ulmoides leaves was extracted by cellulase method. The extraction technology was optimized using the content of Aucubin as index. The antibacterial activity of Aucubin was determined.
RESULTS
The results showed that the optimum technology was as follows; The solid-liquid ratio was 1:12, extracted for 50 min by 0.4% enzyme at 50 degrees C in pH 6.0. The extraction rate of Aucubin was as high as 17.892 mg/g. The Aucubin extracted could obviously inhibit the growth of Escherichia coli and Staphylococcus aureus, the MIC of Aucubin against Staphylococcus aureus and Escherichia coli were 4.832 mg/mL and 9.664 mg/mL respectively. However, Aucubin presented weak inhibitory effect on Streptococcus pneumonia and MG-hemolytic streptococcus, the MIC of Aucubin against them were all 28.946 mg/mL.
CONCLUSIONS
The extraction technology obtained in this experiment is reasonable and feasible with high extraction rate, and the Aucubin has some antibacterial activity.
