Qualitative analysis of the acetogenins from Annona coriacea (Annonaceae) leaves by HPLC-Q-Orbitrap and their antibacterial potential against oral pathogens

Araticum is an edible and appreciable fruit of Annona coriacea, which is popularly known as a traditional herb in the Brazilian cerrado. A phytochemical study from the leaves of A. coriacea showed that HPLC-ESI-Q-Orbitrap® provided through PRM experiments (MS²) is an efficient method for the fast and accurate analysis of a complex mixture of annonaceous acetogenins, with the identification of sylvaticin and gigantetrocin-A type acetogenins for the first time. In addition, the crude leaf extract and acetogenin-rich fractions were assayed against Streptococcus mutans, S. mitis, S. sanguinis and S. salivarius strains, which are usually related to oral infections.

Keywords: Annona coriacea; Acetogenin; ESI-HRMS; HPLC; antibacterial activity.