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Stranica 1 iz 28 rezultatima
[The effect of hypoxia and of diphazine on the acetone bodies content, the intensity of acetylation and the tissue activity of hexokinase].
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[Formation and oxidation of acetone bodies in the tissues and the change in the content of glycogen and lactic acid in acute hypoxia].
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Effects of tetrodotoxin and anaesthetics on brain metabolism and transport during anoxia.
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1. Tetrodotoxin, at concentrations at which it abolishes generation of action potentials in the nervous system, enhances by about 300% the rate of anaerobic glycolysis of brain-cortex slices from adult rats, or from adult and infant guinea pigs. This occurs to a greater extent in Ca(2+)-deficient
Immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole using "in vivo cryotechnique".
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To evaluate hypoxic cells in live mouse liver tissues, immunohistochemistry for protein adducts of reductively activated pimonidazole (PARaPi) was performed using the "in vivo cryotechnique (IVCT)" followed by freeze-substitution fixation. This method was used because cryotechniques have some merits
Hypoxia-ischemia induces a rapid elevation of ubiquitin conjugate levels and ubiquitin immunoreactivity in the immature rat brain.
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Postnatal rats at 7 and 21 days of age were subjected to unilateral hypoxia-ischemia (H/I) by right carotid artery ligation followed by 1.5 to 2 hours of hypoxia (8% oxygen). Brains were frozen at specific intervals of recovery from 0 to 24 hours. Western blots of samples of right and left forebrain
Hypoxia does not promote naphthalene bioaccumulation in the brown shrimp, Penaeus aztecus.
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Since increased ventilation is known to be a common strategy used by aquatic animals to cope with hypoxia, we tested in present study the hypothesis that hypoxia can promote the bioaccumulation of naphthalene, a representative polycyclic aromatic hydrocarbon (PAH), in Penaeus aztecus, a penaeid
Monoacylglycerol lipase. Regulation and increased activity during hypoxia and ischemia.
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The presence of monoacylglycerol lipase was established in extracts of acetone-dried powders from rat and bovine brains using thioester substrate analogs. At pH 7.4, the apparent Km and Vmax values for 1-S-decanoyl-1-mercapto-2,3-propanediol were 56 microM and 227 nmol/h/mg protein in bovine gray
Orobanone analogues from acid-promoted aromatization rearrangement of curcumol inhibit hypoxia-inducible factor-1 (HIF-1) in cell-based reporter assays.
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In this paper, the mechanism of orobanone analogues formation via aromatization rearrangement of curcumol was minutely explored. Aromatization of curcumol with acetone under acidic condition was selected as the model reaction. The formation of a stable aromatic system was the driving force for this
Root anoxia effects on physiology and emissions of volatile organic compounds (VOC) under short- and long-term inundation of trees from Amazonian floodplains.
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Volatile organic compound (VOC) emissions are affected by a variety of biotic and abiotic factors such as light intensity, temperature, CO2 and drought. Another stress factor, usually overlooked but very important for the Amazon region, is flooding. We studied the exchange of VOCs in relation to CO2
Enhanced lipolysis of myocardial triglycerides during low-flow ischemia and anoxia in the isolated rat heart.
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We studied lipolysis in the isolated rat heart, measured as glycerol release during anoxia, low-flow ischemia and subsequent reperfusion. It was found that the rate of lipolysis was enhanced during ischemia/anoxia while the lipase activities in tissue extracts involved in the myocardial lipolysis
Honeycomb-like periodic porous LaFeO₃ thin film chemiresistors with enhanced gas-sensing performances.
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The use of composite materials and polynary compounds is a promising strategy to promote conductometric sensor performances. The perovskite oxides provide various compositional combinations between different oxides for tuning gas-sensing reaction and endowing rich oxygen deficiencies for preferable
[Characteristics of impaired gas exchange in patients with chronic obstructive pulmonary disease].
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Eleven patients with chronic obstructive pulmonary disease (COPD), placed in a supine position, were given a mixture of 0.1% CO and 21% O2 in N2 as inspired gas and normal saline containing the six inert gases including SF6, ethane, cyclopropane, halothane, diethyl ether and acetone via the
Immunolocalization of phospho-Arg-directed protein kinase-substrate in hypoxic kidneys using in vivo cryotechnique.
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Protein kinases (PKs) phosphorylate proteins at active regions for signal transduction. In this study, normal and hypoxic mouse kidneys were prepared using an "in vivo cryotechnique" (IVCT) and examined immunohistochemically with specific antibodies against phospho-(Ser/Thr) PKA/C substrate (P-PK-S)
Recent development of in vivo cryotechnique to cryobiopsy for living animals.
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Various microscopic methods have been used to analyze the morphology and molecular distribution of cells and tissues. Using conventional procedures, however, ischemic or anoxic artifacts are inevitably caused by tissue-resection or perfusion-fixation. The in vivo cryotechnique (IVCT) was developed
Evidence of ultracytochemical mitochondria-derived hydrogen peroxide activity in myocardial cells from broiler chickens with an ascites syndrome.
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The location of the cytotoxic reactive oxygen metabolite hydrogen peroxide was investigated ultracytochemically in broiler chickens which had been reared at sea level and had spontaneous hypoxia-induced ascites syndrome. In the ascitic birds the activity was located within the mitochondrial matrices
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