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[Induced expression of Arabidopsis thaliana WUSCHEL in Escherichia coli, affinity protein purification and polyclonal antibody preparation].

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We constructed a His-tagged prokaryotic expression vector of WUSCHEL gene of Arabidopsis thaliana, pET-31b(+)-WUS-His(6). The induction condition of the fusion protein expression in Escherichia coli was optimized. After purified by affinity chromatography, the recombinant WUS protein was resolved by

Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient-agar media.

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We have characterized the growth responses of Arabidopsis thaliana seedlings to water deficit. To manipulate the water potential, we developed a method whereby the nutrient-agar medium could be supplemented with polyethylene glycol (PEG 8000); PEG was introduced into gelled media by diffusion, which

Omeprazole Enhances Mechanical Stress-Induced Root Growth Reduction in Arabidopsis thaliana.

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Mechanical sensing is one of the most fundamental processes for sessile plants to survive and grow. The response is known to involve calcium elevation in the cell. Arabidopsis seedlings grown horizontally on agar plates covered with a dialysis membrane show a 2-fold reduction in root growth compared

Ethylene signaling plays a pivotal role in mechanical-stress-induced root-growth cessation in Arabidopsis thaliana.

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Plant roots show growth cessation as a primary response to mechanical stress. To clarify the molecular basis of this response, we have previously established an assay system to monitor the root growth response of Arabidopsis seedlings to mechanical stimuli using dialysis membrane-covered agar media.

How many salicylic acid receptors does a plant cell need?

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The small-molecule hormone salicylic acid (SA) is a plant immune signal for which the receptors have only recently been identified. Two recent studies reported that the transcriptional coactivator nonexpresser of pathogenesis-related genes 1 (NPR1) and its paralogues NPR3 and NPR4 are bona fide SA

Arabidopsis GLP4 is localized to the Golgi and binds auxin in vitro.

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Hormones are critical for cell differentiation, elongation, and division. The plant hormone auxin plays vital roles in plant growth and development and is essential for various physiologic processes. Previous studies showed that germin-like proteins (GLPs) are involved in multiple physiologic and

Comparative biochemical characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolates.

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The defensive function of the glucosinolate-myrosinase system in plants of the order Capparales results from the formation of isothiocyanates when glucosinolates are hydrolysed by myrosinases upon tissue damage. In some glucosinolate-containing plant species, as well as in the insect herbivore

Genetic dissection of hormonal responses in the roots of Arabidopsis grown under continuous mechanical impedance.

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We investigated the role of ethylene and auxin in regulating the growth and morphology of roots during mechanical impedance by developing a new growing system and using the model plant Arabidopsis (Arabidopsis thaliana). The Arabidopsis seedlings grown horizontally on a dialysis membrane-covered

Beneficial Rhizobacterium Bacillus amyloliquefaciens SQR9 Induces Plant Salt Tolerance through Spermidine Production.

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The inoculation of plants with plant-growth-promoting rhizobacterium has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress; however, the signal transmitted from bacteria to the plant under salt stress is poorly

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