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Stranica 1 iz 67 rezultatima
Respiration of Sugars in Spinach (Spinacia oleracea), Maize (Zea mays), and Chlamydomonas reinhardtii F-60 Chloroplasts with Emphasis on the Hexose Kinases.
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The role of hexokinase in carbohydrate degradation in isolated, intact chloroplasts was evaluated. This was accomplished by monitoring the evolution of 14CO2 from darkened spinach (Spinacia oleracea), maize (Zea mays) mesophyll, and Chlamydomonas reinhardtii chloroplasts externally supplied with
Alterations in Carbohydrate Intermediates in the Endosperm of Starch-Deficient Maize (Zea mays L.) Genotypes.
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Metabolite levels in kernels of selected starch-deficient mutants of maize (Zea mays L.) were investigated to gain insight into partitioning of carbohydrate metabolism during kernel development. Several free sugars, hexose phosphates, triose phosphates, fructose-2,6-bisphosphate, and pyrophosphate
Distinguishing Biologically Relevant Hexoses by Water Adduction to the Lithium-Cationized Molecule.
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A method to distinguish the four most common biologically relevant underivatized hexoses, d-glucose, d-galactose, d-mannose, and d-fructose, using only mass spectrometry with no prior separation/derivatization step has been developed. Electrospray of a solution containing hexose and a lithium salt
Separation and characterization of four hexose kinases from developing maize kernels.
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Four forms of hexose kinase activity from developing maize (Zea mays L.) kernels have been separated by ammonium sulfate precipitation, gel filtration chromatography, blue-agarose chromatography, and ion exchange chromatography. Two of these hexose kinases utilized d-glucose most effectively and are
H Efflux and Hexose Transport under Imposed Energy Status in Maize Root Tips.
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The relationship between changes in H(+) flux and sugar transport in maize Zea mays L. DEA root tips have been investigated using two methods for controlling the cellular nucleotide level: (a) incubation in the presence of a glucose analog, the 2-deoxyglucose, which decreased the ATP level to less
Enzymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize.
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Tissue distribution and activity of enzymes involved in sucrose and hexose metabolism were examined in kernels of two inbreds of maize (Zea mays L.) at progressive stages of development. Levels of sugars and starch were also quantitated throughout development. Enzyme activities studied were:
Subcellular distribution and kinetic properties of cytosolic and non-cytosolic hexokinases in maize seedling roots: implications for hexose phosphorylation.
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Hexose phosphorylation by hexokinases plays an important role in glycolysis, biosynthesis and control of sugar-modulated genes. Several cytosolic hexokinase and fructokinase isoforms have been characterized and organelle-bound hexokinases have also been detected in higher plants. In this study a
Adaptation of colonic uptake of hexoses and lipids following ileal resection: effect of variations in the fat content of the diet.
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This study was undertaken to determine the effect of varying the dietary composition of fat (corn oil and lard) on the colonic uptake of lipids (medium-chain-length fatty acids, cholesterol and decanol) and hexoses (D-glucose, galactose and 3-O-methyl glucose) in control rabbits with an intact
Effects of ophiobolin a on ion leakage and hexose uptake by maize roots.
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Ophiobolin A, a sesterterpene metabolite of Helminthosporium maydis, Nisikado and Miyake, stimulates net leakage of electrolytes and glucose from maize (Zea mays L.) seedling roots. Treatment of the roots with ophiobolin A at a concentration of 10 mug/ml (25 mum) inhibits uptake of 10 mm
Measurement of Metabolites Associated with Nonaqueously Isolated Starch Granules from Immature Zea mays L. Endosperm.
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Starch granules with associated metabolites were isolated from immature Zea mays L. endosperm by a nonaqueous procedure using glycerol and 3-chloro-1,2-propanediol. The soluble extract of the granule preparation contained varying amounts of neutral sugars, inorganic phosphate, hexose and triose
Duplicated chromosome segments in maize (Zea mays L.): further evidence from hexokinase isozymes.
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The genetic control of hexokinase isozymes (ATP: d-hexose-6-phosphotransferase, E.C. 2.7.7.1, HEX) in maize (Zea mays L.) was studied by starch gel electrophoresis. Genetic analysis of a large number of inbred lines and crosses indicates that the major isozymes observed are encoded by two nuclear
Increasing leaf export and grain import capacities in maize plants under water stress
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The export rate and the carbohydrate concentration were measured in maize plants submitted to water deprivation either at the fourth leaf stage or at pollination. Export rate was evaluated by a short pulse of labelling with 14CO2 followed by a 10-h chase. In stressed plants,
Enhancement of Anaerobic Respiration in Root Tips of Zea mays following Low-Oxygen (Hypoxic) Acclimation.
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Root tips (10-millimeter length) were excised from hypoxically pretreated (HPT, 4% [v/v] oxygen at 25 degrees C for 16 hours) or nonhypoxically pretreated (NHPT, 40% [v/v] oxygen) maize (Zea mays) plants, and their rates of respiration were compared by respirometry under aerobic and anaerobic
Movement of C-Labeled Assimilates into Kernels of Zea mays L: II. Invertase Activity of the Pedicel and Placento-Chalazal Tissues.
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Invertases of the placento-chalazal and pedicel tissues are much more active than invertase from the pericarp of Zea mays L. kernels 12 to 40 days after pollination. Sucrose synthetase was not detected in the pedicel or placento-chalazal tissues. Sucrose content and percentage increased in the
Transport and Metabolism of a Sucrose Analog (1'-Fluorosucrose) into Zea mays L. Endosperm without Invertase Hydrolysis.
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1'-Fluorosucrose (FS), a sucrose analog resistant to hydrolysis by invertase, was transported from husk leaves into maize (Zea mays L., Pioneer Hybrid 3320) kernels with the same magnitude and kinetics as sucrose. (14)C-Label from [(14)C]FS and [(14)C]sucrose in separate experiments was distributed
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