hyoscyamus albus/skopolamin

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A small-scale proteomic approach reveals a survival strategy, including a reduction in alkaloid biosynthesis, in Hyoscyamus albus roots subjected to iron deficiency.

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Hyoscyamus albus is a well-known source of the tropane alkaloids, hyoscyamine and scopolamine, which are biosynthesized in the roots. To assess the major biochemical adaptations that occur in the roots of this plant in response to iron deficiency, we used a small-scale proteomic approach in which

Putrescine and putrescine N-methyltransferase in the biosynthesis of tropane alkaloids in cultured roots of Hyoscyamus albus : II. Incorporation of labeled precursors.

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[1,4-(14)C]Putrescine, administered to cultured roots of Hyoscyamus albus L., was converted to spermidine and spermine as well as to conjugated forms. A substantial fraction of its radioactivity (approx. 6%) was, however, incorporated into N-methylputrescine within 4 h, followed by an increase in

Effect of ontogeny on the content of the hallucinogenic alkaloids atropine and scopolamine in the different organs of some Solanaceae plants

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The content of atropine and scopolamine is known to vary with ontogeny and plant organs selected which makes it necessary to define the optimal stage for harvesting of each plant organ. The present study aims at investigating the effect of ontogeny on the accumulation of atropine and scopolamine in

Putrescine N-Methyltransferase in Cultured Roots of Hyoscyamus albus: n-Butylamine as a Potent Inhibitor of the Transferase both in Vitro and in Vivo.

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Biosynthesis of tropane alkaloids is thought to proceed by way of the diamine putrescine, followed by its methylation by putrescine N-methyltransferase (PMT; EC 2.1.1.53). High PMT activities were found in branch roots and/or cultured roots of several solanaceous plants. PMT was partially purified

Phytochemical investigation of Hyoscyamus albus.

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The work presented in this paper illustrates the isolation and structure elucidation of secondary metabolites of Hyoscyamus albus. Two new natural source and three known compounds were isolated from the Hyoscyamus albus. Among the isolated compounds, grivilloside H (1) and betulaplatoside (2) were

Capillary electrophoresis for the analysis of tropane alkaloids: pharmaceutical and phytochemical applications.

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Three capillary electrophoresis methods, using UV detection, were developed for the simultaneous determination of several tropane alkaloids, including atropine, scopolamine and synthetic derivatives. After optimization, the validated capillary zone electrophoresis methods were applied to the

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